钌–生物质碳人工酶的制备及在比色检测杀虫剂毒死蜱残留中的应用  

Ruthenium-biocarbon Mimic Enzyme:Synthesis and Application in Colorimetric Detection of Pesticide Chlorpyrifos

作　　者：曹志军 李在均 CAO Zhijun;LI Zaijun(School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学化学与材料工程学院,无锡214122

出　　处：《无机材料学报》2022年第5期554-560,共7页Journal of Inorganic Materials

基　　金：国家重点研发计划(2018YFC1603001)。

摘　　要：金属纳米材料人工酶以其高稳定性和低成本的特点而被广泛用于生物传感与催化领域。本研究采用[1-甲基-3-乙基咪唑][二氰胺]离子液体([EMIM][DCA])与氯化钌反应形成[EMIM]_(3)[Ru(DCA)_(6)]离子液体,将蚕丝溶解于[EMIM]_(3)[Ru(DCA)_(6)]后经高温碳化得到钌–生物质碳纳米材料。结果显示,钌–生物质碳纳米材料具有优异的分散性,平均直径为7.5 nm的钌纳米粒子可均匀地分散在碳片表面,表现出较强的类氧化酶活性。基于杀虫剂毒死蜱对钌–生物质碳酶活性的抑制作用,建立了一种测定毒死蜱的比色方法。钌–生物质碳酶能催化3,3′,5,5′-四甲基联苯胺氧化生成蓝色产物。毒死蜱能抑制钌–生物质碳人工酶的活性,从而导致蓝色产物的吸光度下降。当毒死蜱浓度在10~80 ng/mL之间,反应体系的吸光度随毒死蜱浓度增大而线性下降,检出下限为6.5 ng/mL。本方法的灵敏度和稳定性明显优于现有技术,已成功应用于桃果实中毒死蜱农药残留的快速测定。Metal nanomaterials as mimic enzyme have been widely used for biosensor and catalysis due to their high stability and low cost.Here,[1-methyl-3-ethylimidazole][dicyandiamide]ionic liquid([EMIM][DCA])reacted with ruthenium chloride to form[EMIM]_(3)[Ru(DCA)_(6)]ionic liquid.Silk was dissolved in the above ionic liquid and then carbonized at high temperature to obtain Ru-biomass carbon composite.The results show that Ru-biomass carbon offers excellent dispersion,small Ru nanoparticles with an average diameter of 7.5 nm which were uniformly dispersed on the surface of carbon sheets,and high oxidase-like activity.Based on the inhibition of chlorpyrifos on catalytic activity of Ru-biomass carbon,a method was established for colorimetric determination of chlorpyrifos.Chlorpyrifos obviously inhibits the catalytic activity of Ru-biochar carbon,leading to a reduced absorbance of blue product.When the concentration of chlorpyrifos was in the range from 10 to 80 ng/mL,the peak absorbance decreases linearly with the increase of chlorpyrifos concentration,giving a detection limit of about 6.5 ng/mL.The as-proposed method provides higher sensitivity and stability compared with present methods,and has successfully applied to detection of chlorpyrifos in peach samples.

关 键 词：金属离子液体 钌纳米粒子 类氧化酶 有机磷杀虫剂 比色检测 

分 类 号：TQ174[化学工程—陶瓷工业]