不同针灸方法对KOA模型大鼠软骨损伤生物标志物MMP-1、MMP-3和TIMP-1的影响  

Effects of Different Acupuncture Methods on MMP-1,MMP-3 and TIMP-1 of Cartilage Injury in KOA Model Rats

作　　者：任菁钰 王顺 牟秋杰 田会玲[1] 杨佳一 刘浩 汪子栋 李志刚[1] REN Jingyu;WANG Shun;MOU Qiujie;TIAN Huiling;YANG Jiayi;LIU Hao;WANG Zidong;LI Zhigang(Beijing University of Chinese Medicine, Beijing 100029, China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《针灸临床杂志》2022年第6期62-68,共7页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然科学基金重大项目,编号:81590952。

摘　　要：目的:观察比较不同针灸方法对KOA模型大鼠MMP-1、MMP-3和TIMP-1的影响。方法:雄性SD大鼠50只,随机分为正常组(N)、模型组(M)、温针组(W)、电针组(D)和手针组(S)5组,每组10只。N组不作处理,其余4组采用单碘乙酸盐法(MIA)右膝关节注射造模,周期14 d,造模前后进行Lequesne MG评分检测。N、M组不作干预,其余3组对鹤顶、阳陵泉穴分别进行针刺、电针或温针干预。干预前后进行机械刺激回缩阈值检测,28 d后麻醉取材,取关节和关节软骨进行HE、免疫组化和Western blot检测。结果:Lequesne MG评分检测显示,造模前各组关节评分差异无统计学意义(P>0.05),造模后除N组外,其余4组评分显著高于造模前,差异具有统计学意义(P<0.05)。机械刺激回缩阈值检测显示,干预前M、W、D和S组较N组均降低(P<0.05),干预后D组较M组升高,差异具有统计学意义(P<0.05)。HE染色软骨形态显示N组结构完整,M组结构破坏严重,W、S和D组结构较完整。免疫组化检测关节软骨显示,MMP-1阳性表达M组较N组升高,差异具有统计学意义(P<0.05),D、W和S组较M组降低,差异具有统计学意义(P<0.05),D组较W组降低,差异具有统计学意义(P<0.05);MMP-3阳性表达M组较N组升高,差异具有统计学意义(P<0.05),D、W和S组较M组降低,差异具有统计学意义(P<0.05),D组较S和W组降低,差异具有统计学意义(P<0.05);T1MP-1阳性表达N、M、W和S组较D组降低,差异具有统计学意义(P<0.05),S组较M组升高,差异具有统计学意义(P<0.05)。Western blot检测软骨显示,MMP-1蛋白表达M组较N组升高,差异具有统计学意义(P<0.05),D、W和S组较M组降低,差异具有统计学意义(P<0.05),W组较D组升高,差异具有统计学意义(P<0.05);MMP-3蛋白表达M组较N组升高,差异具有统计学意义(P<0.05),D、S组较M组降低,差异具有统计学意义(P<0.05);T1MP-1蛋白表达D、S和W组较M组升高,差异具有统计学意义(P<0.05)。结论:电针、温针和手Objective:To observe the effects of different acupuncture methods on MMP-1,MMP-3 and TIMP-1 in model rats of knee osteoarthritis(KOA).Methods:50 male SD rats were randomly divided into the normal group,the model group,the needle-warming acupuncture group,the electro-acupuncture group and the hand acupuncture group,with 10 rats in each group.Monoiodoacetate(MIA)was injected into right knee joint to establish KOA model for 14 days.The behavioral Lequesne MG score was tested before and after modeling.Heding(EX-LE2)and Yanglingquan(GB34)were chosen in the hand acupuncture group,the electro-acupuncture group and the needle-warming acupuncture group.All the three groups were treated every other day for 28 days.Behavioral mechanical stimulation retraction threshold was detected before and after the intervention.The samples of articular cartilage and joints were collected for HE staining,Western blot detection and immunohistochemical detection after the intervention.Results:In terms of Lequesne MG score test,there was no statistical difference in the knee joint score among all groups before modeling(P>0.05),which was significantly higher after modeling compared with that before modeling(P<0.05).In terms of mechanical stimulation retraction threshold,it was lower in the model group,the needle-warming acupuncture group,the electro-acupuncture group and the hand acupuncture group compared with that in the normal group(P<0.05);after intervention,it was higher in the electro-acupuncture group than that in the model group(P<0.05).HE staining of the cartilage showed that the structure was complete in the normal group,the it was seriously damaged in the model group,and it was fair complete in the needle-warming acupuncture group,the electro-acupuncture group and the hand acupuncture group.Immunohistochemical results showed that the positive expression of MMP-1 was higher in the model group than that in the normal group(P<0.05),it was lower in the electro-acupuncture group,the needle-warming acupuncture group and the hand acupunc

关 键 词：基质金属蛋白酶-1 基质金属蛋白酶-3 基质金属蛋白酶抑制剂-1 膝骨关节炎 针灸 

分 类 号：R246.2[医药卫生—针灸推拿学]