“原络通经”针法对SAMP8小鼠病理形态学及NMDAR表达的影响  

Effect of‘Yuanluo Tongjing’Acupuncture on Pathomorphology and NMDAR Expression in SAMP8 Mice

作　　者：郭文海[1] 颜敬彧 邢菁[1] 陈奥[1] 徐佳[1] 马伯艳[2] 史珊怡[1] GUO Wenhai;YAN Jingyu;XING Jing;CHEN Ao;XU Jia;MA Baiyan;SHI Shanyi(The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150001, China;Heilongjiang University of Chinese Medicine, Harbin 150040, China)

机构地区：[1]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《针灸临床杂志》2022年第6期69-74,共6页Journal of Clinical Acupuncture and Moxibustion

基　　金：黑龙江省自然科学基金,编号:H2018061;黑龙江省中医药科研项目,编号:ZHY19-049。

摘　　要：目的:观察“原络通经”针法对SAMP8小鼠海马CA-1区病理形态学及NMDAR表达的影响,探讨“原络通经”针法治疗阿尔茨海默病(AD)的潜在作用机制。方法:将30只SAMP8雄性8月龄小鼠随机分为SAMP8空白对照组、SAMP8+“原络通经”针法治疗组以及SAMP8+百会穴针刺组,将10只SAMR1小鼠作为SAMR1正常对照组。其中SAMP8+“原络通经”针法治疗组选择丰隆穴、太白穴、关元穴及百会穴针刺,SAMP8+百会穴针刺组选择百会穴针刺。SAMP8+“原络通经”针法治疗组以及SAMP8+百会穴针刺组均针刺1次/d,共干预30 d,每7 d休息1次。比较4组小鼠的学习记忆能力、海马CA-1区病理损伤程度以及NMDAR表达水平。结果:HE染色结果提示,SAMP8对照组出现核皱缩及空泡,相比于SAMP8对照组,SAMP8+“原络通经”针法治疗组和SAMP8+百会穴针刺组在干预后改善明显,未见严重的病理损伤。Western Blot检测结果提示,相比于SAMP8对照组,SAMP8+“原络通经”针法治疗组和SAMP8+百会穴针刺组的NMDAR表达水平显著降低。结论:“原络通经”针法可以改善SAMP8小鼠海马CA-1区病理损伤程度,进而对AD产生治疗效应,其机制可能与下调NMDAR表达有关。Objective:To observe the effect of‘Yuanluo Tongjing’acupuncture on pathomorphology and NMDAR expression in hippocampal CA-1 region of SAMP8 mice,and to explore the potential mechanism of acupuncture in treating Alzheimer’s disease(AD).Methods:30 SAMP8 male 8-month-old mice were randomly divided into SAMP8 blank control group,SAMP8 plus‘Yuanluo Tongjing’acupuncture group,and SAMP8 plus Baihui(DU20)acupuncture group.Another 10 SAMR1 mice were chosen as normal control.SAMP8 plus‘Yuanluo Tongjing’acupuncture group was treated with needling Fenglong(ST40),Taibai(SP3),Guanyuan(RN4)and DU20;SAMP8 plus DU20 acupuncture group was treated with needling DU20;both groups were treated once per day for 30 days in total,with one day interval between every 7 days.The ability of learning and memory,the degree of pathological damage in the CA-1 region of hippocampal and the expression of NMDAR were compared among the four groups.Results:HE staining results showed that nuclear shrinkage and vacuoles appeared in SAMP8 control group.Compared with SAMP8 control group,SAMP8 plus‘Yuanluo Tongjing’acupuncture group and SAMP8 plus DU20 acupuncture group improved significantly after intervention,and no serious pathological damage was found.Western Blot results showed that compared with SAMP8 control group,the expression of NMDAR was significantly lower in SAMP8 plus‘Yuanluo Tongjing’acupuncture group and SAMP8 plus DU20 acupuncture group.Conclusion:‘Yuanluo Tongjing’acupuncture can improve pathological damage degree of hippocampal CA-1 area of SAMP8 mice,and then has a therapeutic effect on AD,and its mechanism may be related to down-regulation of NMDAR expression.

关 键 词：阿尔茨海默病 SAMP8小鼠 N-甲基-D-天冬氨酸受体 原络通经针法 

分 类 号：R246.6[医药卫生—针灸推拿学]