182例患者乙肝病毒基因分型与原发性肝癌的关系  

Relationship between hepatitis B virus genotyping and primary hepatocellular carcinoma of 182 patients

作　　者：王欣[1,2] 宋乐 方昕 章必成 WANG Xin;SONG Le;FANG Xin;ZHANG Bi-cheng(Wuhan University,Wuhan,Hubei 430071,China;Department of Infection,Wuhan Yaxin General Hospital,Wuhan,Hubei 430010,China;Department of Infection,PLA Central Theater General Hospital,Wuhan,Hubei 430070,China;Cancer Center,Wuhan University People′s Hospital,Wuhan,Hubei 430060,China)

机构地区：[1]武汉大学,湖北武汉430071 [2]武汉亚心总医院感染科,湖北武汉430010 [3]解放军中部战区总医院感染内科,湖北武汉430070 [4]武汉大学人民医院肿瘤中心,湖北武汉430060

出　　处：《公共卫生与预防医学》2022年第4期141-143,共3页Journal of Public Health and Preventive Medicine

摘　　要：目的分析武汉地区乙肝病毒基因分型与原发性肝癌(PHC)的关系,为PHC的早期预防和诊断提供理论依据。方法选取2020年2月至2021年2月来武汉亚心总医院治疗的慢性乙型肝炎(CHB)患者,根据是否合并原发性肝癌分为PHC组(182例)与对照组(189例),所有研究对象在入院时抽取空腹时静脉血5mL,采用实时荧光定量PCR法测定乙肝HBV基因分型;采用荧光探针杂交技术及PCR扩增技术测定CHB病毒DNA,并根据其产物测序分析来进行基因分型及检测耐药突变点。采用Spearman直线相关分析PHC患者基因分型及突变率与PHC的相关性研究。结果PHC组患者C基因型比例高于非PHC组患者(P<0.05);两组患者B基因型和B、C基因混合型比例间差异无统计学意义(P>0.05);PHC组乙肝病毒突变型比例(114/182)高于对照组(84/189)(χ^(2)=12.331,P<0.05);两组在HBV B型和B、C混合型突变型比例间差异无统计学意义P>0.05);PHC组HBV C型突变型比例高于对照组(P<0.05);PHC组患者HBV C型突变型比例高于HBV B型和B、C混合型(P<0.05);Spearman相关分析显示C基因型、C基因型突变率与PHC的发生呈正相关,相关性系数为(r_(1)=0.349,r_(2)=0.305,P<0.05)。结论PHC患者HBV基因型主要为C型,并且具有较高的C基因型突变率,在临床上可通过检测CHB的基因分型及C基因型突变率用于PHC的诊断。Objective To analyze the relationship between hepatitis B virus genotyping and primary liver cancer(PHC)in Wuhan,and to provide a theoretical basis for the early prevention and diagnosis of PHC.Methods Patients with chronic hepatitis B(CHB)from Wuhan Sub-heart General Hospital for treatment from February 2020 to February 2021 were selected and divided into PHC group(182 cases)and control group(189 cases)according to whether they were complicated with primary liver cancer.5ml of fasting elbow venous blood was taken from all subjects at admission.HBV genotyping was determined by real-time fluorescence quantitative PCR.The DNA of CHB virus was determined by fluorescence probe hybridization and PCR amplification,and genotyping and drug-resistant mutation points were detected according to the product sequencing analysis.Spearman linear correlation analysis was used to analyze the correlation between genotyping and mutation rate of PHC patients.Results The proportion of C genotype in PHC group was significantly higher than that in non-PHC group(P<0.05).There was no significant difference in the proportion of B genotype and mixed B and C genotype between 2 groups(P>0.05).The proportion of HEPATITIS B virus mutation in PHC group(114/182)was significantly higher than that in control group(84/189)(χ^(2)=12.331,P<0.05).There was no significant difference in the proportion of HBV B type and B,C mixed mutation between the two groups(P>0.05).The proportion of HBV C mutant in PHC group was significantly higher than that in control group(P<0.05).The proportion of HBV C mutation in PHC group was significantly higher than that of HBV B and B,C mixed type(P<0.05).Spearman correlation analysis showed that the mutation rate of C genotype and C genotype was positively correlated with the occurrence of PHC,and the correlation coefficients were(r_(1)=0.349,r_(2)=0.305,P<0.05).Conclusion The HBV genotype of PHC patients is mainly TYPE C,and has a high mutation rate of C genotype.It can be used for diagnosis of PHC by detecting the genot

关 键 词：乙肝病毒基因分型 原发性肝癌 突变率 

分 类 号：R181[医药卫生—流行病学]