拟水狼蛛对镉和Bt蛋白联合作用的响应  

Response of Pirata subpiraticus to combined effect of cadmium and Bacillus thuringiensis protein

作　　者：雷梓妍 吕波 王智 LEI Ziyan;LYU Bo;WANG Zhi(College of Resources and Environment,Hunan Agricultural University,Changsha,Hunan 410128,China;College of Life Science,Hunan Normal University,Changsha,Hunan 410081,China)

机构地区：[1]湖南农业大学资源与环境学院,湖南长沙410128 [2]湖南师范大学生命科学学院,湖南长沙410081

出　　处：《湖南农业大学学报（自然科学版）》2022年第3期305-311,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(31472017、31272339);湖南省科学技术厅项目(2014FJ2003);湖南省教育厅重点项目(18A024)。

摘　　要：用经镉(1.0mg/L)、Bt(Bacillusthuringiensis)蛋白(0.1mg/L)和Cd+Bt处理的果蝇饲喂拟水狼蛛(Pirata subpiraticus),测定拟水狼蛛体内Cd与Bt蛋白的富集状况和抗氧化酶表达水平;利用RNA-seq技术分析Cd+Bt处理拟水狼蛛的差异表达基因(DEGs),运用生物信息学方法构建DEGs的富集及调控通路。结果发现:与Cd和Bt处理的拟水狼蛛(Cd质量分数为(6.965±0.21)mg/kg、Bt质量分数为(3.82±0.28)μg/kg)相比,Cd+Bt处理的拟水狼蛛具有更高的Cd质量分数((12.23±0.86)mg/kg)和Bt质量分数((7.98±0.49)μg/kg)(P<0.05);与对照组、单一Cd和单一Bt处理的拟水狼蛛的相比,Cd+Bt处理还能提高拟水狼蛛体内的谷胱甘肽过氧化物酶、谷胱甘肽S-转移酶和超氧化物歧化酶的活性(P<0.05);通过对Cd+Bt处理和对照组进行转录组测序,得到6914条差异表达基因,并且与肽链内切酶活性、激素生物合成以及信息素代谢过程相关的基因(519条)都显著下调,共有14条参与卵母细胞减数分裂的基因在Cd+Bt处理下显著下调。转录因子和蛋白质互作分析表明,ZBTB等转录因子参与胁迫相关的调控网络,主要影响遗传信息的转录和翻译过程。Cadmium-treated(1.0 mg/L),Bt-treated(Bacillus thuringiensis protein,0.1 mg/L),and Cd+Bt-treated flies were used as food for Pirata subpiraticus(P.subpiraticus),and the enrichment of Cd and Bt protein and expression of antioxidant enzymes in the spiders were investigated.In addition,RNA-seq was employed to analyze the differentially expressed genes(DEGs)of spiders under Cd+Bt treatment,and the enrichment and regulation pathways of DEGs was constructed using bioinformatics methods.It is found that compared with Cd(Cd content in the spiders was(6.965±0.21 mg/kg)and Bt(Bt content in the spiders was(3.82±0.28)μg/kg groups,the spiders in the Cd+Bt group showed higher Cd(12.23±0.86)mg/kg and Bt(7.98±0.49)μg/kg contents(P<0.05).In addition,compared with control,Cd and Bt groups,the Cd+Bt treatment increased the activities of glutathione peroxidase,glutathione S-transferase,and superoxide dismutase in spiders(P<0.05).RNA-seq was conducted on spiders in Cd+Bt treatment and in the control,a total of 6914 DEGs were obtained,and it is found that genes(519)involved in endopeptidase activity,hormone biosynthesis,and pheromone metabolism were all down-regulated obviously.Fourteen genes involved in oocyte meiosis were significantly down-regulated under Cd+Bt treatment.Through the analysis of transcription factors and protein interactions,we also found that transcription factors such as ZBTB participate in stress-related regulatory networks,which mainly affect the transcription and translation process of genetic information.

关 键 词：拟水狼蛛 镉 苏云金芽孢杆菌蛋白 抗氧化酶 转录组 基因调控 

分 类 号：Q959.226[生物学—动物学]