基于DNA-AuNCs内滤效应诱导荧光猝灭灵敏检测4-硝基苯酚  

DNA-Au Nanoclusters Inner Filter Effect Induced Fluorescence Quenching for Sensitive Detection of 4-Nitrophenol

作　　者：王海波[1] 陶贝贝 吴宁宁 毛安丽 张红定 WANG Haibo;TAO Beibei;WU Ningning;MAO Anli;ZHANG Hongding(College of Chemistry and Chemical Engineering/Xinyang Key Laboratory of Functional Nanomaterials for Bioanalysis,Xinyang Normal University,Xinyang 464000,China)

机构地区：[1]信阳师范学院化学化工学院/信阳市功能纳米材料生物分析重点实验室,河南信阳464000

出　　处：《信阳师范学院学报（自然科学版）》2022年第3期464-468,共5页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(U1704153);河南省高层次人才特殊支持中原千人计划—中原青年拔尖人才(ZYQR201912153);河南省高等学校青年骨干教师培养计划(2017GGJS100)。

摘　　要：以A30 DNA作为合成模板,制备了荧光DNA模板金纳米簇(DNA-AuNCs),其最大激发波长为290 nm,最大发射波长为475 nm。当4-硝基苯酚(4-NP)存在时,其紫外吸收光谱与DNA-AuNCs的荧光激发光谱和发射光谱均有部分重叠,发生内滤效应,使DNA-AuNCs的荧光信号被显著地猝灭。基于内滤效应诱导DNA-AuNCs荧光信号猝灭,构建了一种灵敏检测4-NP的荧光分析方法。该方法对4-NP的线性检测范围为1~80μmol/L,检出限为0.3μmol/L(R_(SN)=3)。此外,该方法被成功应用于湖水样品中4-NP含量的测定。DNA-templated Au nanoclusters(DNA-AuNCs)are synthesized by using A30 DNA as the templates.The obtained DNA-AuNCs possess a maximum excitation wavelength at 290 nm and a maximum emission wavelength at 475 nm.In the presence of 4-nitrophenol,the fluorescence signal of DNA-AuNCs is significantly quenched by the inner filter effect,due to the UV absorption spectrum of 4-NP overlaped partly with the fluorescence excitation and emission spectra of DNA-AuNCs.A novel fluorescent strategy is developed for the sensitive determination of 4-nitrophenol(4-NP)based on the DNA-AuNCs inner filter effect(IFE)induced fluorescence quenching.The method exhibits good linear relationship with 4-NP concentrations in the range from 1~80μmol/L with the limit of detection of 0.3μmol/L(R_(SN)=3).In addition,the method is successfully applied for the determination of 4-NP content in lake water samples.

关 键 词：DNA模板金纳米簇 内滤效应 4-硝基苯酚检测 荧光传感新方法 

分 类 号：O657.39[理学—分析化学]