The intestinal microbiome of an Indo-Pacific humpback dolphin(Sousa chinensis)stranded near the Pearl River Estuary,China  

作　　者：Xiaoling WAN Jia LI Zhaolong CHENG Mengxue AO Renmao TIAN Richard William MCLAUGHLIN Jinsong ZHENG Ding WANG 

机构地区：[1]The Key Laboratory of Aquatic Biodiversity and Conservation of the Chinese Academy of Sciences,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,China [2]University of Chinese Academy of Sciences,Beijing,China [3]Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,China [4]Institute for Environmental Genomics,Department of Microbiology and Plant Biology,School of Civil Engineering and Environmental Sciences,University of Oklahoma,Norman,Oklahoma,USA [5]Institute for Food Safety and Health,Illinois Institute of Technology,Bedford Park,Illinois,USA [6]General Studies,Gateway Technical College,Kenosha,Wisconsin,USA

出　　处：《Integrative Zoology》2021年第3期287-299,共13页整合动物学（英文版）

基　　金：This research was supported by grants from the Bu-reau of Science&Technology for Development,the Chinese Academy of Sciences(No.ZSSD-004);the National Key Programme of Research and Develop-ment of Ministry of Science and Technology of China(2016YFC0503200).

摘　　要：The mammalian intestinal microbiome is critical for host health and disease resistance.However,the cetacean intestinal microbiota remains relatively unexplored.By using high-throughput 16S rRNA gene sequencing,we analyzed intestinal bacterial samples from an Indo-pacific humpback dolphin(Sousa chinensis)stranded near the Pearl River Estuary in China.The samples included 3 anatomical regions(foregut,midgut,and rectum)and 2 anatomical locations(content and mucus).Our analyses revealed that the dolphin intestinal bacteria contained 139 operational taxonomic units(OTUs),dominated at the phyla level by Firmicutes(47.05%in the content;94.77%in the mucus),followed by Bacteroidetes(23.63%in the content;1.58%in the mucus)and Gammaproteobacteria(14.82%in the content;2.05%in the mucus).The intestinal bacteria had a small core community(15 OTUs,accounting for 99.74%of the reads),some of which could be potentially pathogenic to both human and dolphins.As an alternative to sampling the dolphin intestinal bacteria,fecal sampling could be used.Additionally,function potentials such as,xenobiotics biodegradation,beta-lactam resistance,and human disease-related pathways,were detected in the dolphin intestinal bacteria.These findings provide the first baseline knowledge of the intestinal microbiome of the Indo-Pacific humpback dolphin,which may offer new insights into cetacean conservation by using microbial surveillance.

关 键 词：16S rRNA gene high-throughput sequencing anthropogenic impact CETACEAN indo-pacific humpback dolphin intestinal microbiota 

分 类 号：Q93[生物学—微生物学]