Genetic diversity,phylogenetic position and morphometric analysis of Astacus colchicus(Decapoda,Astacidae):a new insight into Eastern European crayfish fauna  

作　　者：Martin BLÁHA JiríPATOKA Bella JAPOSHVILI Marek LET MilošBURˇICˇ Antonín KOUBA Levan MUMLADZE 

机构地区：[1]Faculty of Fisheries and Protection of Waters,South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses,University of South Bohemia in CˇeskéBudeˇjovice,Vodnˇany,Czech Republic [2]Department of Zoology and Fisheries,Faculty of Agrobiology,Food and Natural Resources,Czech University of Life Sciences Prague,Praha,Czech Republic [3]Institute of Zoology of Ilia State University,Tbilisi,Georgia

出　　处：《Integrative Zoology》2021年第3期368-378,共11页整合动物学（英文版）

基　　金：The study was financially supported by the Ministry of Education,Youth and Sports of the Czech Republic(projects CENAKVA II–LO1205 under the NPU I program)。

摘　　要：The phylogeny of European crayfish fauna,especially with respect to Eastern European species,is still far from being completely resolved.To fill this gap,we analyzed most of the European crayfish species focusing on the phylogenetic position of the endemic crayfish Astacus colchicus,inhabiting Georgia.Three mitochondrial and one nuclear marker were used to study evolutionary relationships among European crayfish species,resulting in the unique phylogenetic position of A.colchicus indicating independent species status to A.astacus.Phylogenetic analyses revealed a deep molecular divergence of A.colchicus in comparison to A.astacus(6.5–10.9%in mtDNA and 1.1%in nDNA)as well as to Pontastacus leptodactylus and P.pachypus(5.5–10.0%in mtDNA and 1.4–2.4%in nDNA).Absent ventral process on second male pleopod and abdominal somites II and III with pleura rounded lacking prominent spines clearly indicate taxonomic assignment to the genus Astacus;however,the species is distributed almost in the middle of Ponto-Caspian area typical by occurrence of the genus Pontastacus.Several morphological indices linked to head length,carapace,and total body length and width were found to demonstrate apparent differences between A.colchicus and A.astacus.Although this study provides a novel insight into European crayfish phylogeography,we also point out the gaps in comprehensive study of the P.leptodactylus species complex,which could reveal details about the potential species status of particular species and subspecies within this genus.

关 键 词：12S rRNA 16S rRNA cytochrome c oxidase histone H3 Ponto-Caspian crayfish 

分 类 号：Q95[生物学—动物学]