A reassessment of the diversity of green toads(Bufotes)in the circum-Aegean region  

作　　者：Christophe DUFRESNES Nikolas MPROBONAS Ilias STRACHINIS 

机构地区：[1]LASER,College of Biology and the Environment,Nanjing Forestry University,Nanjing,People’s Republic of China [2]Filoti,Naxos,Greece [3]Department of Genetics,Development and Molecular Biology,School of Biology,Faculty of Natural Sciences,Aristotle University of Thessaloniki,Thessaloniki,Greece

出　　处：《Integrative Zoology》2021年第3期420-428,共9页整合动物学（英文版）

基　　金：Most of the data combined in this paper was originally published by Dufresnes et al.(2018,2019b);who made it available on dryad(doi:10.5061/dryad.6qv8666);NCBI SRA(BioProject PRJNA542138),respectively。

摘　　要：The dynamic biogeography of glacial refugia may cause complex patterns of genetic admixture between parapatric taxa,which in turn can mislead their systematics,diversity,and distributions.We investigated this issue for green toads(Bufotes)inhabiting the circum-Aegean region,a biodiversity hotspot of the Eastern Mediterranean.A previous phylogeographic study based on mitochondrial and microsatellite loci identified the hybrid zone between the European(viridis)and Anatolian(sitibundus)lineages of B.viridis all over the Balkan Peninsula,but subsequent range-wide genomic analyses(>1000 SNPs)located this transition in Turkey,a thousand kilometers eastwards.In order to clarify the diversity and taxonomy of the circum-Aegean populations,we reconciled these conflicting findings by integrating previous data with pure sitibundus individuals.Our results confirmed that the viridis/sitibundus hybrid zone extends in Western Anatolia,but that southeastern European populations feature cytonuclear discordances and a high and structured microsatellite diversity.This remarkable situation may stem from a massive geographic displacement of the hybrid zone during the last glacial fluctuations,an underappreciated yet seemingly common feature among the herpetofauna of the region.Our study thus contributes to the rising view that mitochondrial DNA can be a poor predictor of current phylogeographic structure,hence the need for genomic data,especially for narrowly distributed taxa.Finally,the analyses unambiguously support the distinction of a micro-endemic clade of green toads unique to some Cyclades islands,for which we provide a formal taxonomic description.

关 键 词：CYCLADES hybrid zone NAXOS PHYLOGEOGRAPHY SPECIATION 

分 类 号：Q95[生物学—动物学]