Temperature and mass scaling affect cutaneous and pulmonary respiratory performance in a diving frog  

作　　者：Ryan B.MCWHINNIE Jason P.SCKRABULIS Thomas R.RAFFEL 

机构地区：[1]Department of Biological Sciences,Oakland University,Rochester,Michigan,USA

出　　处：《Integrative Zoology》2021年第5期712-728,共17页整合动物学（英文版）

基　　金：supported by an NSF-CAREER grant to T.R.R.(IOS-1651888).

摘　　要：Global climate change is altering patterns of temperature variation,with unpredictable consequences for species and ecosystems.The Metabolic Theory of Ecology(MTE)provides a powerful framework for predicting climate change impacts on ectotherm metabolic performance.MTE postulates that physiological and ecological processes are limited by organism metabolic rates,which scale predictably with body mass and temperature.The purpose of this study was to determine if different metabolic proxies generate different empirical estimates of key MTE model parameters for the aquatic frog Xenopus laevis when allowed to exhibit normal diving behavior.We used a novel methodological approach in combining a flow-through respirometry setup with the open-source Arduino platform to measure mass and temperature effects on 4 different proxies for whole-body metabolism(total O_(2) consumption,cutaneous O_(2) consumption,pulmonary O_(2) consumption,and ventilation frequency),following thermal acclimation to one of 3 temperatures(8℃,17℃,or 26℃).Different metabolic proxies generated different mass-scaling exponents(b)and activation energy(EA)estimates,highlighting the importance of metabolic proxy selection when parameterizing MTE-derived models.Animals acclimated to 17℃ had higher O_(2) consumption across all temperatures,but thermal acclimation did not influence estimates of key MTE parameters EA and b.Cutaneous respiration generated lower MTE parameters than pulmonary respiration,consistent with temperature and mass constraints on dissolved oxygen availability,SA:V ratios,and diffusion distances across skin.Our results show that the choice of metabolic proxy can have a big impact on empirical estimates for key MTE model parameters.

关 键 词：African clawed frog AMPHIBIAN pneumotachography thermal biology 

分 类 号：Q95[生物学—动物学]