手十二井穴放血激活TRPV1通道保护心脏骤停后综合征大鼠中枢神经损伤  

Pricking Blood on Twelve Hand Jing-Well Acupoints in Protecting Central Nerve Injury in Rats with PCAS via Activating TRPV1 Pathway

作　　者：钟晓芃[1] 郭义[2] ZHONG Xiaopeng;GUO Yi(Tianjin People's Hospital,Tianjin 300122,China;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津市人民医院,天津300122 [2]天津中医药大学,天津301617

出　　处：《针灸临床杂志》2022年第5期59-65,共7页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然科学基金重点项目,编号:82030125;天津市卫健委课题,编号:2019121。

摘　　要：目的:探讨手十二井穴放血疗法对心脏骤停后综合征大鼠中枢神经保护作用及其机制。方法:24只大鼠随机分为:(1)假手术组(Control组,6只),只行气管插管,不诱导心脏骤停和进行心肺复苏(CPR);(2)模型组(Model组,6只),气管夹闭窒息法诱导心脏骤停后进行常规CPR;(3)手十二井穴放血组(HTWP组,6只)气管夹闭窒息法诱导心脏骤停后进行常规CPR,复苏后1 h、24 h及48 h进行手十二井穴放血;(4)辣椒平组(CPZ组,6只)于气管夹闭窒息法诱导心脏骤停前45 min经腹腔注射辣椒平20 mg/kg,心脏骤停后进行常规CPR,复苏后1 h、24 h、48 h进行手十二井穴放血。于复苏后72 h进行神经功能缺陷评分,纸带移除实验,取静脉血ELISA法测定S100β,取海马组织尼氏染色检测神经细胞损伤,TUNEL法测定神经细胞凋亡,Western blot检测大鼠海马CA1区TRPV1、Caspase3及Blc-2表达。结果:复苏后72 h,神经功能缺陷评分Control组评分多于其他3组,差异具有统计学意义(P<0.05),HTWP组评分多于Model组及CPZ组,差异有统计学意义(P<0.05)。纸带移除实验Control组用时少于其他3组,差异有统计学意义(P<0.05),HTWP组用时少于Model组及CPZ组,差异有统计学意义(P<0.05)。尼氏染色HTWP组大鼠脑组织神经元水肿程度较轻,神经元形态较好,仅见少量空泡样改变,有部分神经元细胞核肿大,核仁消失。Control组血清S100β水平虽然低于HTWP组但差异无统计学意义(P=0.219 5),HTWP组的血清S100β水平低于Model组和CPZ组,差异有统计学意义(P<0.05)。TUNEL方法分析各组大鼠海马神经细胞凋亡情况表明:心脏骤停后综合征使大鼠海马神经元发生凋亡,而HTWP组细胞凋亡计数较Model组、CPZ组更少,差异有统计学意义(P<0.05)。Western blot结果显示:HTWP组的TRPV1表达明显高于其他3组,差异有统计学意义(P<0.01);HTWP组的Blc-2表达明显高于其他3组,差别有统计学意义(P<0.01);Control组与HTWP组Caspase-3表达相似,Objective: To explore the protective effect and mechanism of pricking blood on twelve hand Jing-well acupoints on the central nervous system in rats with post-cardiac arrest syndrome(PCAS). Methods: 24 male SD rats were randomly divided into the control group(n=6), the model group(n=6), the HTWP group(n=6) and the CPZ group(n=6). The control group was only received endotracheal intubation without inducing cardiac arrest and cardiopulmonary resuscitation(CPR), the model group was performed routine CPR after cardiac arrest induced by trachea clipping and asphyxia, the HTWP was performed routine CPR after cardiac arrest induced by trachea clamping and asphyxia followed by pricking blood on twelve hand Jing-well acupoints 1 h, 24 h and 48 h after resuscitation, and the CPZ group was preprocessed with intraperitoneal injection of Capsicum 45 min before cardiac arrest, followed by routine CPR after cardiac arrest and pricking blood on twelve hand Jing-well acupoints 1 h, 24 h and 48 h after resuscitation. 72 hours after resuscitation, the neurological severity scores were assessed and paper tape removal experiment was carried out, venous blood was collected for S100β detection by ELISA method, the nerve cell injury was detected by Nissl’s staining in the hippocampal tissue, the nerve cell apoptosis was determined by TUNEL method, and the expressions of TRPV1, Caspase3 and BLC-2 in the hippocampal CA1 region of rats were detected by Western blot. Results: 72 hours after resuscitation, the scores of neurological deficits were significantly higher in the control group than those in the other three groups(P<0.05), and the scores in the HTWP group were significantly higher than those in the model group and the CPZ group(P<0.05). The duration of the paper tape removal experiment in the control group was less than that in the other three groups(P<0.05), and the duration of the HTWP group was less than that in the model group and the CPZ group(P<0.05). The neuronal edema in the brain tissues of the rats was relatively mild

关 键 词：手十二井穴 放血 瞬时受体电位感受器1 心脏骤停后综合征 

分 类 号：R246.1[医药卫生—针灸推拿学]