DSA引导下食蟹猴大脑中动脉栓塞模型的建立与评价  

Establishment of DSA-guided middle cerebral artery embolization mode in cynomolgus monkeys and its technical evaluation

作　　者：叶娟 尚海龙[1] 沈海林[1] 杜红娣[1] 沈萍[1] 朱枫[1] 邱志富 曹莹 赵芳红 王莺[1] YE Juan;SHANG Hailong;SHEN Hailin;DU Hongdi;SHEN Ping;ZHU Feng;QIU Zhifu;CAO Ying;ZHAO Fanghong;WANG Ying(Department of Medical Imaging,Suzhou Kowloon Hospital,School of Medicine,Shanghai Jiao Tong University,Suzhou,Jiangsu Province 215028,China)

机构地区：[1]上海交通大学医学院苏州九龙医院医学影像科,江苏215028 [2]上海浦灵生物科技有限公司 [3]上海交通大学医学院苏州九龙医院放疗科

出　　处：《介入放射学杂志》2022年第4期369-373,共5页Journal of Interventional Radiology

摘　　要：目的 建立并评价DSA导引下食蟹猴大脑中动脉闭塞(MCAO)模型。方法 纳入15只成年雄性食蟹猴。DSA导引下经股动脉切开通过导管向大脑中动脉(MCA)注入猴自体静脉血栓凝块构建MCAO模型。术后分别行颅脑MRI和头颈部CTA+CT灌注(CTP)检查,以明确MCA梗塞后影像学变化。术后7 d连续观察实验猴行为表现,于术后第8天处死,取脑组织行三苯基四唑氯染色。结果 15只实验猴中1只术前头颈部CTA提示颅内血管畸形,未行手术。14只接受手术的猴中12只经DSA、头颈部CTA+CTP及颅脑MRA扫描证实MCA闭塞成功;1只术中死亡;另1只栓塞后DSA及CT、MRI扫描均显示无明显缺血,麻醉复苏后无脑缺血行为表现。12只实验猴术后均存活,神经功能评分提示有神经功能缺损,其缺损变化与影像学改变相符。结论 DSA导引下将自体静脉血栓引入MCA建立的食蟹猴MCAO模型具有手术创伤小、可重复性高、稳定性强的特点。Objective To establish an middle cerebral artery occlusion(MCAO) model under the guidance of digital subtraction angiography(DSA) in cynomolgus monkeys,and to evaluate its technical advantages.Methods A total of 15 adult male cynomolgus monkeys were included in this study.Under the guidance of DSA,the femoral artery incision with catheter insertion was performed,which was followed by catheterization of middle cerebral artery(MCA) and injection of autologous venous clot into MCA to establish MCAO model.After modeling,brain MRI scan,head and neck computed tomography angiography(CTA) and CT perfusion(CTP) scan were adopted to check the cerebral imaging changes after MCA was occluded.The postoperative behavior of the experimental cynomolgus monkeys was kept under continuous observation for consecutive 7days.The experimental monkeys were sacrificed on the 8th day after operation,and the brain tissues of monkeys were taken for triphenyltetrazolium chloride(TTC) staining.Results Among the 15 experimental animals,one had intracranial vascular malformations,which was suggested by preoperative head and neck CTA and surgery was not adopted.Of the 14 experimental animals receiving surgery,successful MCA occlusion was achieved in 12,which was confirmed by DSA,head and neck CTA plus CTP,and cranial MRA scan.One animal died during operation;in another animal post-embolization DSA,CT and MRI showed no obvious signs of ischemia,and no abnormal behaviors due to cerebral ischemia were observed after anesthesia resuscitation.Twelve experimental monkeys survived after surgery,and the evaluation score of neurological function suggested that there was neurological impairment,and the impairment changes were consistent with the imaging findings.Conclusion The technique used for establishing MCAO model in cynomolgus monkeys,i.e.DSA-guided injection of autologous venous clot into MCA,has the advantages of small surgical trauma,high repeatability,and strong stability of model.(J Intervent Radiol,2022,31:369-373)

关 键 词：食蟹猴 DSA导引 大脑中动脉闭塞 动物模型 影像评价 

分 类 号：R332[医药卫生—人体生理学]