西藏黄绿卷毛菇生境土壤微生物群落组成  

Composition of soil microbial community in the habitat of Floccularia luteovirens in Tibet,southwest China

作　　者：任丽莹 白玛央宗 丹增晋美 刘晓玲[3,5] 宗同铠 刘淑艳 刘小勇 普布多吉[4] REN Liying;PEMA Yangzom;TENZIN Jigme;LIU Xiaoling;ZONG Tongkai;LIU Shuyan;LIU Xiaoyong;PHURBU Dorji(College of Plant Protection,Jilin Agricultural University,Changchun 130118,Jilin,China;Engineering Research Center of Edible and Medicinal Fungi,Ministry of Education,Jilin Agricultural University,Changchun 130118,Jilin,China;State Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;Tibet Plateau Institute of Biology,Lhasa 850000,Tibet,China;College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049,China;College of Biodiversity Conservation,Southwest Forestry University,Kunming 650224,Yunnan,China;College of Life Sciences,Shandong Normal University,Jinan 250014,Shandong,China)

机构地区：[1]吉林农业大学植物保护学院,吉林长春130118 [2]吉林农业大学食药用菌教育部工程研究中心,吉林长春130118 [3]中国科学院微生物研究所真菌学国家重点实验室,北京100101 [4]西藏自治区高原生物研究所,西藏拉萨850000 [5]中国科学院大学生命科学学院,北京100049 [6]西南林业大学生物多样性保护学院,云南昆明650224 [7]山东师范大学生命科学学院,山东济南250014

出　　处：《菌物学报》2022年第6期906-917,共12页Mycosystema

基　　金：西藏自治区科技计划项目(XZ201901-GB-19);国家自然科学基金(31970009)。

摘　　要：牧草外生菌根菌黄绿卷毛菇Floccularia luteovirens具有较高的生态和经济价值,其生境微生物对其菌丝生长、菌根化和子实体形成具有促进作用。本研究利用高通量测序技术对西藏黄绿卷毛菇菌窝土壤及其周围无菇土壤微生物群落组成进行分析,挖掘促进黄绿卷毛菇生长的有益微生物资源。结果显示,西藏黄绿卷毛菇生境土壤细菌隶属于17门22纲116目161科227属,其中酸杆菌门、变形菌门、拟杆菌门和疣微菌门累计比例高达81.75%;生境土壤真菌隶属于5门17纲34目55科61属,子囊菌门和担子菌门累计比例高达80.89%。对比周围无菇土壤的微生物群落,促进黄绿卷毛菇生长的潜在细菌类群为Flavisolibacter、黄杆菌属Flavobacterium、芽单胞菌属Gemmatimonas、Haliangium、赛格特杆菌Segetibacter和鞘氨醇单胞菌属Sphingomonas;促进黄绿卷毛菇生长的潜在真菌类群为斜盖伞属Clitopilus、丝膜菌属Cortinarius、被孢霉属Mortierella和毛霉属Mucor。土壤微生物促进黄绿卷毛菇生长繁殖的机制有待进一步研究。Floccularia luteovirens,an ectomycorrhizal fungus in grassland,is highly valuable to ecology and economy.Herbage rhizosphere microorganisms can promote the growth of ectomycorrhizal fungi,mycorrhizal formation and the formation of fruiting bodies.In this study,high-throughput sequencing technology was applied to analyze the microbial community composition in fruiting body-emersed and surrounding soil of F.luteovirens in Tibet,attempting to explore beneficial microorganism resources for promoting the fungal growth.The results showed that bacteria in the soil of F.luteovirens habitat could be referred to 17 phyla,22 classes,116 orders,161 families and 227 genera,and the dominant phyla Acidobacteria,Proteobacteria,Bacteroidetes and Verrucomicrobia accounted for 81.75%.Fungi in the habitat soil were referred to 5 phyla,17 classes,34 orders,55 families and 61 genera,and dominant phyla Ascomycota and Basidiomycota accounted for 80.89%.The bacteria potentially promoting the growth of F.luteovirens were Flavisolibacter,Flavobacterium,Gemmatimonas,Haliangium,Segetibacter and Sphingomonas;the fungi potentially promoting the growth of F.luteovirens were Clitopilus,Cortinarius,Mortierella and Mucor.The mechanisms of soil microorganisms promoting the growth and reproduction of F.luteovirens need further study.

关 键 词：黄绿蜜环菌 宏基因组 外生菌根菌 rDNA小亚基 rDNA转录间隔区 

分 类 号：S154.3[农业科学—土壤学] S646[农业科学—农业基础科学]