枸杞LbMYB12基因的克隆及表达特性分析  

Gene Cloning and Temporal-spatial Expression Analysis of LbMYB12 in Wolfberry(Lycium barbarum L.)

作　　者：冯嘉馨 刘普浩 张兴 黑晓萍 岳思君[1,2] 郑国琦 毛桂莲[1,3] 姚新灵[1,2,3] 郑蕊[1,2,3] Feng Jiaxin;Liu Puhao;Zhang Xing;Hei Xiaoping;Yue Sijun;Zheng Guoqi;Mao Guilian;Yao Xinling;Zheng Rui(College of Life Science,Ningxia University,Yinchuan,750021;Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Ningxia University,Yinchuan,750021;Key Laboratory of Modem Molecular Breeding for Dominant and Special Crops in Ningxia,Ningxia University,Yinchuan,750021)

机构地区：[1]宁夏大学生命科学学院,银川750021 [2]宁夏大学,西部特色生物资源保护与利用教育部重点实验室,银川750021 [3]宁夏大学,宁夏优势特色作物现代分子育种重点实验室,银川750021

出　　处：《分子植物育种》2022年第12期3913-3920,共8页Molecular Plant Breeding

基　　金：宁夏自然科学基金项目(2020AAC03097;2018AAC02001);宁夏枸杞产业发展中心项目(HSZB-2020ZC242);宁夏回族自治区重点研发计划(重大)重点项目(2019BBF02022);国家自然科学基金项目(81760682)共同资助。

摘　　要：类黄酮是植物中一类重要的次生代谢产物,枸杞黄酮以其强抗氧化作用以及对心脑血管疾病的抑制和改善功能而广为人知。MYB转录因子参与类黄酮代谢过程的调控。本研究采用RACE技术克隆了枸杞LbMYB12基因全长cDNA序列。生物信息学分析表明,LbMYB12基因的完整开放阅读框(ORF)长930 bp,具有MYB蛋白家族保守结构域,蛋白质相对分子量为35.56 kD,等电点为5.05,其二级结构主要为α-螺旋。荧光定量PCR分析表明,LbMYB12基因在枸杞整个植株中均有表达。其中,在根中的表达量最低,在青果中的表达量最高。LbMYB12基因在果实不同发育时期的表达分析表明,开花后早期表达量显著高于成熟期果实。本研究为进一步探讨枸杞LbMYB12转录因子在类黄酮代谢中的作用提供了研究基础。Flavonoids a re a cla ss of important secondary metabolites in plants.Flavonoids,as one of the main active components,have been paid more and more attention in wolfberry.The MYB transcription factor is involved in regulating the metabolism of flavonoids.In this study,the full length cDNA of LbMYB12 gene was cloned by RACE technology.Bioinformatics analysis showed that the complete open reading frame(ORF)of LbMYB12 was930 bp with the conserved domain of MYB protein family,the relative molecular weight of the protein coded by this gene was 35.56 k D,the isoelectric point was 5.05,and its secondary structure was mainly composed ofα-helix.Fluorescence quantitative PCR analysis showed that LbMYB12 gene was expressed in the whole plant.Among them,the gene expression level in root was the lowest and the highest level was in green fruit.Analysis of LbMYB12gene expression during different development of fruit showed that the expression level of LbMYB12 in early period after flowering was significantly higher than that of maturity.This study provided a reference for further study on the role of Lb MYB12 transcription factor in flavonoid metabolism.

关 键 词：枸杞 LbMYB12转录因子 基因克隆 表达分析 

分 类 号：S567.19[农业科学—中草药栽培]