烟草骨干亲本马铃薯Y病毒病抗性鉴定及抗性来源分析  

Identification and Source Analysis of Resistance to Potato Virus Y in Tobacco Foundation Parents

作　　者：杨晓宁 龚敏 张新要 王东 蒋彩虹[1] 程立锐[1] 刘旦[1] 杨爱国[1] Yang Xiaoning;Gong Min;Zhang Xinyao;Wang Dong;Jiang Caihong;Cheng Lirui;Liu Dan;Yang Aiguo(Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao,266101;Com Research Institute,Sichuan Agricultural University,Chengdu,610000;China Tobacco Hunan Industrial Co.,Ltd.,Changsha,410007)

机构地区：[1]中国农业科学院烟草研究所,青岛266101 [2]四川农业大学玉米研究所,成都610000 [3]湖南中烟工业有限责任公司,长沙410007

出　　处：《分子植物育种》2022年第12期3988-3997,共10页Molecular Plant Breeding

基　　金：中国烟草总公司烟草基因组计划重大专项(110202001019[JY-02]);湖南中烟工业有限责任公司科技项目(201943000834049)共同资助。

摘　　要：马铃薯Y病毒病(potato virus Y,PVY)是影响烟草产量和质量的主要病毒病害,选育抗病品种是解决病毒病害经济有效、环境友好的方法,而丰富抗病亲本的遗传多样性在品种选育中具有非常重要的作用。为深入认识PVY抗性种质的抗性来源,选取30份烟草(Nicotiana tabacum)骨干亲本进行人工接种鉴定其PVY抗性,筛选出11份抗PVY烟草种质。为进一步探究材料的抗性来源,基于感病基因eIF4E1开发了PVY1分子标记,并利用VAM和K326构建的F2遗传群体对该标记进行了验证。用此标记对骨干亲本进行检测,结果发现Y48等6份抗病种质中无法扩增出条带,而CV58等5份抗病种质中可以扩增出大小为348 bp的条带,进一步分析发现CV58中eIF4E1基因并未发生突变。以上结果表明Y48等对PVY的抗性来源于eIF4E1基因的缺失,而CV58等具有新的抗性位点,其对PVY的抗性并非来源于eIF4E1基因的缺失或突变。本研究获得了两种不同抗性来源的抗PVY烟草种质资源,为烟草PVY抗性品种的选育提供借鉴。Potato virus Y(PVY)is a major virus disease that affects tobacco yield and quality.Breeding resistant varieties is an economical,effective,and environment-friendly solution to conquer virus diseases.Enriching the genetic diversity of resistant parents plays an important role in breeding.To understand the source of resistance to PVY in tobacco germplasm,30 tobacco(Nicotiana tabacum)foundation parents were used to evaluate PVY resistance by artificial inoculation in the green house,and 11 cultivars showed resistance to PVY.To further explore the source of resistance,a PVY1 molecular marker was developed based on the susceptible gene eIF4E1,and then verified by F2genetic population constructed by VAM and K326.Using this marker to detect the foundation parents,no bands were amplified in 6 resistant varieties including Y48,while the 348 bp bands were amplified in another five resistant varieties including CV58.Further analysis showed that eIF4E1 gene in CV58did not mutate.These results indicated that the resistance of Y48 to PVY is due to the deletion of eIF4E1 gene,while CV58 is not from the deletion or mutation of the gene.In this study,two kinds of PVY resistance sources were identified,which provided reference for the breeding of tobacco varieties with PVY resistance.

关 键 词：烟草(Nicotiana tabacum) PVY病毒 抗性鉴定 抗性来源 分子标记 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]