对羟基苯甲酸对广藿香幼苗根系抗氧化酶及土壤酶活性的影响  

Effects of p-Hydroxybenzoic Acid on the Antioxidant Enzyme and Rhizosphere Soil Enzyme Activities of Patchouli Seedlings

作　　者：曹诗佳 严武平 姚广龙[1] 于靖[1] 杨东梅[1] 张军锋[1] 杨华庚 吴友根[1] Cao Shijia;Yan Wuping;Yao Guanglong;Yu Jing;Yang Dongmei;Zhang Junfeng;Yang Huageng;Wu Yougen(College of Horticulture,Hainan University,Haikou,570228)

机构地区：[1]海南大学园艺学院,海口570228

出　　处：《分子植物育种》2022年第12期4094-4100,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(81860681);海南省自然科学基金高层次人才项目(2019RC087);海南省研究生创新科研课题(Hys2020-237)共同资助。

摘　　要：为阐明在酚酸类化感自毒物质对羟基苯甲酸(4-HBA)胁迫下,广藿香幼苗根系生理响应及根际土壤酶活性变化,实验研究了5种不同浓度(0,0.5,1.0,5.0,10.0 mmol/L)的4-HBA处理下,广藿香幼苗抗氧化保护酶(SOD,CAT和POD)活性、丙二醛(MDA)、可溶性蛋白含量和根际土壤酶活性的变化,从植物生理抗性方面探讨4-HBA胁迫下盆栽广藿香幼苗的化感自毒效应。研究结果显示,随着4-HBA处理浓度升高,广藿香幼苗地下部鲜重呈下降趋势,根系中MDA含量上升,可溶性蛋白含量减少,抗氧化物保护酶(SOD,CAT和POD)活性均出现低浓度时活性上升高浓度时活性下降的现象。广藿香幼苗根际土壤酶活性产生抑制现象,土壤多酚氧化酶、磷酸酶、脲酶、蔗糖酶等酶活性随着处理浓度的升高而下降,而蛋白酶活性会随着浓度处理的增加而增加。本研究从植物生理与土壤环境的角度分析了化感作用对于广藿香幼苗的影响,为广藿香连作障碍的研究提供了理论支持。In order to clarify the effects of phenolic acid allelochemicals p-hydroxybenzoic acid(4-HBA)on the root growth and soil enzyme activities of patchouli potted seedlings and their physiological responses,the antioxidant enzymes(SOD,CAT and POD),malondialdehyde(MDA),soluble protein content and rhizosphere soil enzyme activities of patchouli potted seedlings were studied under five different concentrations(0,0.5,1.0,5.0 and 10.0 mmol/L)of 4-HBA.The allelopathic autotoxic effects of patchouli seedling growth on 4-HBA stress were studied from the perspective of physiological resistance.The results showed that with 4-HBA concentration increased,patchouli seedling underground biomass was on the decline,MDA content increased,soluble protein content decreased,and the root antioxidant enzymes(SOD,CAT and POD)activity showed low promotion and high inhibition.The soil enzyme activity in the rhizosphere of patchouli seedlings was inhibited.The enzyme activities such as soil polyphenol oxidase,phosphatase,urease,and invertase will decrease with the increase of the treatment concentration,while the protease activity will increase with the increase of the concentration treatment.The effects of allelopathic effects on patchouli seedlings were studied from the perspective of plant physiology and soil environment,which provided theoretical support for the research on patchouli continuous cropping obstacles.

关 键 词：广藿香 对羟基苯甲酸 化感物质 土壤酶活性 抗氧化酶 

分 类 号：S567.239[农业科学—中草药栽培]