陆均松花芽分化期蛋白质、碳水化合物及叶绿素变化分析  

Analysis on the Changes of Protein,Carbohydrate and Chlorophyll in Dacrydium pectinatum during Flower Bud Differentiation

作　　者：赵霞 鹿文举 何研涵 曾晓鹏 李一凡 王健[1] 宋希强[1] 田代科 赵莹[1] Zhao Xia;Lu Wenju;He Yanhan;Zeng Xiaopeng;Li Yifan;Wang Jian;Song Xiqiang;Tian Daike;Zhao Ying(Hainan Biological Key Laboratory for Gcrmplasm Resources of Tropical Special Ornamental Plants,Key Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants,College of Forestry,Hainan University,Haikou,570228;Shanghai Key Laboratory of Plant Functional Genomics and Resources,Shanghai Chenshan Botanical Garden,Shanghai,201602;Hainan Tropical Forest Ecology Research Co.Ltd.,Haikou,570228)

机构地区：[1]海南大学林学院,热带特色林木花卉遗传与种质创新教育部重点实验室,海南省热带特色花木资源生物学重点实验室,海口570228 [2]上海辰山植物园上海市资源植物功能基因组学重点实验室,上海201602 [3]海南热带森木林业生态研究有限公司,海口570228

出　　处：《分子植物育种》2022年第12期4101-4107,共7页Molecular Plant Breeding

基　　金：国家自然科学地区基金项目(31760217);海南省自然科学基金高层次人才项目(320RC471);上海市资源植物功能基因组学重点实验室开放课题资助(PFGR202101)共同资助。

摘　　要：为揭示陆均松花芽分化阶段的生理生化变化规律,本研究以不同时期陆均松大小孢子叶球(Dacrydium pectinatum)为材料,测定其在陆均松大、小孢子叶球发育过程中蛋白质、碳水化合物及叶绿素含量的变化。结果表明,在陆均松小孢子叶球花粉母细胞发育前,蛋白质、碳水化合物及叶绿素含量均呈现上升趋势,这说明了在小孢子发育前期,这些指标有利于小孢子叶球的发育。在陆均松大孢子叶球发育期间,可溶性糖和可溶性蛋白含量在胚珠形成期间有上升趋势,该结果说明了可溶性糖和蛋白质含量的增加有利于胚珠的形成。本研究为陆均松花芽分化的分子研究提供了参考基础。In order to reveal the physiological and biochemical changes during the flower bud differentiation of Dacrydium petinatum,determine the changes of protein,carbohydrate and chlorophyll content during the development of Dacrydium petinatum’s large and microspore leaf bulbs.The results showed that,before the development of the pollen mother cell of the spore leaf ball,protein,carbohydrates and chlorophyll all showed an upward trend,which indicated that these indicators were conducive to the development of the microspore leaf ball in the early stage of microspore development.During Dacrydium pectinatum’s megaspore leaf bulb development,the soluble sugar and protein content increased during the ovule formation period.These indicators indicated that the increasing of soluble sugar and protein content were beneficial to the formation of ovules.This study provides a reference basis for the molecular research of Dacrydium pectinatum flower bud differentiation.

关 键 词：陆均松 花芽分化 生理生化指标 

分 类 号：S791[农业科学—林木遗传育种]