盐胁迫和干旱胁迫对青海地理种源麻花艽种子萌发的影响  

Effects of Salt and Drought Stress on Seed Germination of Gentiana straminea from Qinghai Provenances

作　　者：程庭峰 王环[2] 陈世龙[1,3] 王久利 师生波[1,3] 沈建伟[2] 周党卫[1,3,5] Cheng Tingfeng;Wang Huan;Chen Shilong;Wang Jiuli;Shi Shengbo;Shen Jianwei;Zhou Dan-gwei(Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,810008;Analysis and Testing Center,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,810008;Key Laboratory of Crop Molecular Breeding of Qinghai Province,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,810008;The College of Ecological Environment and Resources,Qinghai Minzu University,Xining,810007;University of Chinese Academy of Sciences,Beijing,100049)

机构地区：[1]中国科学院西北高原生物研究所,高原生物适应与进化重点实验室,西宁810008 [2]中国科学院西北高原生物研究所分析测试中心,西宁810008 [3]中国科学院西北高原生物研究所,青海省作物分子育种重点实验室,西宁810008 [4]青海民族大学生态环境与资源学院,西宁810007 [5]中国科学院大学,北京100049

出　　处：《分子植物育种》2022年第12期4170-4176,共7页Molecular Plant Breeding

基　　金：青海省科技厅基础研究项目(2017-ZJ-702;2019-ZJ-7016);青海省自然科学基金项目(2019-ZJ-976Q);青海省创新平台建设专项(2017-ZJ-Y14)共同资助。

摘　　要：以青海省海北站、德令哈、河北乡、下藏科和班玛县5个地理种源麻花艽种子为材料,研究不同浓度NaCl和PEG胁迫下麻花艽种子的萌发率以及150 mmol/L NaCl和10%PEG胁迫下不同种源麻花艽种子的萌发特性。结果显示,麻花艽种子的适宜萌发Na Cl浓度为50 mmol/L,适宜萌发PEG浓度为5%。不同地理种源麻花艽胁迫下的萌发特性具有明显差异,150 mmol/L NaCl胁迫下,河北乡地区麻花艽种子发芽指数最高,为7.33;10%PEG处理下,德令哈地区发芽指数最高,为8.16。通过隶属函数法分析发现,5个地理种源麻花艽种子的耐盐能力依次为:河北乡>下藏科>德令哈>海北站>班玛县;抗旱能力依次为:德令哈>海北站>下藏科>河北乡>班玛县。不同地理种源麻花艽种子对胁迫的耐受性具有差异性。本研究为麻花艽优质种质资源的引种栽培提供了参考。The seeds of Gentiana straminea from five geographical provenances of Qinghai Province,namely Haibeizhan,Delingha,Hebeixiang,Xiazangke and Banmaxian,were used as research materials.To study the germination rate of G.straminea seeds under different concentrations of NaCl and PEG,and the germination characteristics of G.straminea seeds from different provenances under the stress of 150 mmol/L NaCl and 10%PEG.The results showed that the optimal concentration of Na Cl and PEG was 50 mmol/L and 5%,respectively.Meanwhile,in the different conditions of stress,the germination characteristics of G.straminea from different geographical provenances were significantly different.Specifically,under the stress of 150 mmol/L NaCl,the germination index of G.straminea seeds was the highest in Hebeixiang(7.33);the germination index was the highest in Delingha under the 10%drought stress(8.16).According to the comprehensive appraisal of membership function method,the salt tolerance of G.straminea seeds from five geographic provenances ranged from large to small as follows,Hebeixiang>Xiazangke>Delingha>Haibeizhan>Banmaxian;About drought resistance,Delingha>Haibeizhan>Xiazangke>Hebeixiang>Banmaxian.Therefore,we concluded that the tolerance of G.straminea seeds from different geographical provenances has a certain difference.This study provides a theoretical basis for the introduction and cultivation of high-quality germplasm resources of G.straminea.

关 键 词：麻花艽 种子萌发 地理种源 盐胁迫 干旱胁迫 

分 类 号：S567.239[农业科学—中草药栽培]