原电池型氧化还原电位分析法  

Galvanic redox potentiometry

作　　者：薛亦飞 吴菲 蒋亚楠 于萍[2] 毛兰群 Yifei Xue;Fei Wu;Yanan Jiang;Ping Yu;Lanqun Mao(College of Chemistry,Beijing Normal University,Beijing 100875,China;Key Laboratory of Analytical Chemistry for Living Biosystems,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China)

机构地区：[1]北京师范大学化学学院,北京100875 [2]中国科学院化学研究所,活体分析化学院重点实验室,北京100190

出　　处：《中国科学：化学》2022年第6期848-857,共10页SCIENTIA SINICA Chimica

基　　金：国家重点研究发展计划(编号:2018YFE0200800);国家自然科学基金(编号:22134002,21790390,21790391);国家基础研究计划(编号:2016YFA0200104和2018YFA0703501);中国科学院战略重点研究(编号:XDB30000000,QYZDJSSW-SLH030)资助项目。

摘　　要：提出和发展分析化学原理和方法,在整体动物层次实现对脑内神经化学分子的活体实时原位检测,对揭示脑功能及脑疾病的分子机制具有重要意义.电化学方法由于可以实现高的时空分辨率、选择性和灵敏度,在脑化学检测较早得到了关注,并展示出良好的应用前景.然而,随着研究的不断深入,脑神经科学的发展对活体电化学分析的神经元兼容性提出更高的要求,传统基于电解池原理的电化学分析方法由于在测量过程中需要施加极化电压并产生电流,不仅可能会对神经元活动造成影响,而且也会导致其难以与电生理技术联用.针对这一方法学的挑战,近些年来本课题组提出了原电池型氧化还原电位分析法,通过构筑自发的电化学氧化还原过程,并以开路电位作为记录方式实现对电活性神经化学物质的定量分析.该方法具有神经元兼容等优势并对其他电学信号记录无干扰,在脑神经过程研究中具有重要应用价值.本文对原电池型氧化还原电位分析法的提出、方法原理、分析性能和应用等方面进行综述,并对该方法未来的研究重点和发展方向做出了展望.The development of in vivo analytical methods for accurate, efficient, and neuron-compatible measurement of neurochemicals during physiological and pathological processes in living brain has important implications for understanding complex neurophysiological processes and the molecular mechanisms of brain functions. Electrochemical methods have been widely used for the accurate measurement of multiple neurochemicals, which benefits from their high spatio-temporal resolution, selectivity, and sensitivity properties. However, the nervous system is sensitive to electrical stimuli. Therefore, traditional electrochemical measurement process would affect spontaneous neuronal activity in the brain and make it difficult to cooperate with electrophysiological recording. To address it, galvanic redox potentiometry has recently established for neuron-compatible in vivo electrochemical analysis. The method built on galvanic cell configurations bearing the advantages of high neuronal compatibility and no interference to electrical recording. These properties are useful for neurochemical studies. In this review, we summarize the status of in vivo electrochemical analysis, systematically elaborate the recent progress of galvanic redox potentiometry in terms of fundamental principles and practical applications and provide a perspective for the future development.

关 键 词：电化学分析 活体分析化学 原电池型氧化还原电位分析法 

分 类 号：O657.1[理学—分析化学]