两相体系中生物转化肉桂醇生成天然2-苯乙醇  

Biotransformation of cinnamyl alcohol into natural 2-phenylethanol in two-phase system

作　　者：黄秋容 粟桂娇[2] 梁敏 赖芳[1] 刘雄民[1] 马丽[1] HUANG Qiurong;SU Guijiao;LIANG Min;LAI Fang;LIU Xiongmin;MA Li(College of Chemistry and Chemical Engineering,Guangxi University,Nanning 530005,Guangxi,China;College of Life Sciences and Technology,Guangxi University,Narming 530005,Guangxi,China)

机构地区：[1]广西大学化学化工学院,广西南宁530005 [2]广西大学生命科学与技术学院,广西南宁530005

出　　处：《精细化工》2022年第6期1184-1189,共6页Fine Chemicals

基　　金：国家自然科学基金资助项目(21502027);广西自然科学基金资助项目(2016GXNFAA380304)。

摘　　要：利用肉桂内生菌鞘氨醇单胞菌Sphingomonassp.Z45生物转化肉桂醇合成天然2-苯乙醇。为了提高肉桂醇的转化率和产物2-苯乙醇的质量浓度,探究了在油相(有机溶剂)/水相(发酵培养基)两相体系中进行生物转化的工艺。结果表明,在筛选的6种有机溶剂中,油酸作为构建两相体系的有机相,在10 mL发酵培养基中,加入1 mL种子液培养24 h后,同时加入底物肉桂醇3 g/L,油酸10 mL,在30℃、转速为200 r/min的摇床中反应12 h,在该条件下肉桂醇转化率达88.0%,2-苯乙醇质量浓度为2.64 g/L。与单水相体系同等条件下(底物肉桂醇加入量3 g/L)的转化结果相比较,肉桂醇转化率提高了41.2%,2-苯乙醇质量浓度提高了1.43 g/L。Natural 2-phenylethanol was synthesized by biotransformation of cinnamyl alcohol with an endophytic strain Sphingomonas sp. Z45 from cinnamon. To improve the conversion rate of cinnamyl alcohol and the mass concentration of 2-phenylethanol, the biotransformation conditions in organic solvent/water two-phase system were investigated. The results showed that oleic acid was the best organic phase for the two-phase system among 6 kinds organic solvents. When 1 mL seed liquid was inoculated in 10 m L fermentation medium and cultured for 24 h, subsequently, 10 m L oleic acid and 3 g/L cinnamyl alcohol were added, and then the reaction was performed at 30 ℃ and 200 r/min for 12 h, the conversion rate of cinnamyl alcohol reached 88.0%, and the final mass concentration of 2-phenylethyl alcohol was 2.64 g/L. Compared with those of the mono-aqueous system reaction under the same conditions(additive amount of cinnamyl alcohol of 3 g/L), the conversion rate of cinnamyl alcohol and the mass concentration of 2-phenylethanol were increased by 41.2% and 1.43 g/L, respectively.

关 键 词：肉桂醇 天然2-苯乙醇 两相体系 鞘氨醇单胞菌 生物转化 生物工程 

分 类 号：TQ243.4[化学工程—有机化工]