清肝泻肺方治疗肝火犯肺增加流感病毒易感性的作用研究  

Effect of Qinggan Xiefei Fang on increasing susceptibility of influenza virus caused by "liver fire invading lung"

作　　者：陆钰辉 欧阳淑桦 翁竞玉 刘佩[1,4] 陈新星 栗原博 李怡芳[1,2,3] 何蓉蓉[1,2,3] LU Yu-hui;OUYANG Shu-hua;WENG Jing-yu;LIU Pei;CHEN Xin-xing;HIROSHI Kurihara;LI Yi-fang;HE Rong-rong(Guangdong Engineering Research Center of Chinese Medicine and Disease Susceptibility,Jinan University,Guangzhou 510632,China;Institute of Traditional Chinese Medicine and Natural Products,Jinan University,Guangzhou 510632,China;Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research,Jinan University,Guangzhou 510632,China;School of Pharmacy and State Key Laboratory of Quality Research in Chinese Medicine,Macao University of Science and Technology,Macao 999078,China)

机构地区：[1]暨南大学,广东省疾病易感性及中医药研发工程技术研究中心,广东广州510632 [2]暨南大学,中药及天然药物研究所,广东广州510632 [3]暨南大学,广东省中药药效物质基础及创新药物研究重点实验室,广东广州510632 [4]澳门科技大学中医药学院,中药质量研究国家重点实验室,中国澳门999078

出　　处：《药学学报》2022年第6期1649-1656,共8页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(82174054)。

摘　　要：本实验采用慢性拘束应激诱导的H1N1流感病毒易感的小鼠模型,模拟临床上情志郁结、肝火犯肺,研究清肝泻肺方(黛蛤散合黄芩泻白散)治疗流感病毒性肺炎的作用。小鼠感染流感病毒后观察21天内的生存和发病情况,并于感染后的第6天取部分小鼠肺脏检测病毒性肺炎和磷脂过氧化等指标。实验结果显示,清肝泻肺方能够缓解慢性拘束应激负荷H1N1流感病毒引起的小鼠生存率和健康率下降,抑制小鼠肺部流感病毒的复制、炎症因子的产生,降低磷脂过氧化水平,改善小鼠肺炎症状。利用中药网络药理学技术构建的清肝泻肺方活性化合物-作用靶点网络,结果显示,该网络包含171个单体化合物和260个靶点,涉及氧化应激、炎症和免疫等相关信号通路。以上结果表明,清肝泻肺方治疗流感病毒性肺炎的作用机制可能与其调控氧化应激有关。实验方案经暨南大学动物实验伦理委员会批准,所有程序均严格按照动物使用和护理的伦理原则进行。In this study, chronic emotional stress-induced H1N1 influenza susceptibility model was employed to simulate the states of "emotional stagnation" and "liver fire invading lung", and the protective effect of Qinggan Xiefei Fang on viral pneumonia was investigated. Survival rate and morbidity rate of mice were observed within21 days after H1N1 infection, the symptoms of viral pneumonia and the level of phospholipid peroxidation were detected in lungs of mice after 6-day infection. The experimental results showed that Qinggan Xiefei Fang could alleviate the decline of survival rate and morbidity rate of mice caused by chronic constraint stress loaded with H1N1, inhibit the replication of H1N1 and the production of inflammatory factors, reduce the level of phospholipid peroxidation, and improve the symptoms of pneumonia in mice. The results also showed that compound-target network of Qinggan Xiefei Fang contained 171 compounds and 260 corresponding targets involved in the signaling pathway of oxidative stress, inflammation and immunity. All the above results indicate that Qinggan Xiefei Fang protecting influenza virus pneumonia was related to the regulation of oxidative stress. The animal experimental protocol has been reviewed and approved by Laboratory Animal Ethics Committee of Jinan University, in compliance with the Institutional Animal Care Guidelines.

关 键 词：情志应激 清肝泻肺方 流感病毒性肺炎 氧化应激 磷脂过氧化 

分 类 号：R966[医药卫生—药理学]