Visualization of the process:selenocysteine activates GPX4 in ferroptosis based on a nano-fluorescent probe  

作　　者：Dongrui Luan Wenfei Guo Xiaonan Gao Kehua Xu Bo Tang 

机构地区：[1]College of Chemistry,Chemical Engineering and Materials Science,Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong,Key Laboratory of Molecular and Nano Probes,Ministry of Education,Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals,Shandong Normal University,Jinan 250014,China [2]Department of Clinical Laboratory,The Second Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250033,China

出　　处：《Science China Chemistry》2022年第7期1286-1290,共5页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21775091,91753111,21927811);the Key Research and Development Program of Shandong Province(2018YFJH0502)。

摘　　要：Developing accurate methods for visualizing the expressions of proteins during the life process is a hotspot in the biochemical research field.Glutathione peroxidase 4(GPX4),one type of selenoproteins,was previously identified as a significant ferroptosis-regulated protein.Therefore,a novel approach was established to visualize the activation process of selenocysteine(Sec)toward GPX4 via an Au–Se bonded fluorescent nanoprobe and assisted by western blot analysis.The Au–Se nanoprobe was constructed by selenol-modified peptides for monitoring the concentration changes of Sec.The fluorescence intensity of Sec in confocal fluorescence imaging positively correlates with GPX4's activation level,proved by western blot analysis in living cells.This study provides an intuitional and visual strategy for predicting the functional expression of GPX4 during the ferroptosis process and offers new directions for the future investigation of proteins'functional expressions.

关 键 词：ferroptosis nano-fluorescent probe selenocysteine activates GPX4 VISUALIZATION 

分 类 号：Q51[生物学—生物化学]