大豆肽补充量和运动时间对大鼠血清游离氨基酸水平的影响  

Effects of Soybean Peptide Supplement and Exercise Time on Serum-Free Amino Acid Levels in Rats

作　　者：谭秋实 邵晶[1] 周丽丽[1] 高红[1] 许葆华[1] 付劲德[1] 王启荣[1] 方子龙[1] 伊木清[1] Tan Qiushi;Shao Jing;Zhou Lili;Gao Hong;Xu Baohuang;Fu Jingde;Wang Qirong;Fang Zilong;Yi Muqing(National Institute of Sports Medicine,Beijing 100061,China)

机构地区：[1]国家体育运动医学研究所运动营养研究中心,国家运动营养测试研究中心,国家体育总局运动营养重点实验室,北京100061

出　　处：《中国运动医学杂志》2022年第4期288-299,共12页Chinese Journal of Sports Medicine

基　　金：国家重点研发计划课题(2016YFD0400603);可口可乐研究基金。

摘　　要：目的:研究大鼠一次性游泳耐力运动1.5 h和3.0 h中补充不同大豆肽含量饮料对血清游离氨基酸(FAA)水平的影响。方法:110只Wistar大鼠随机分为11组,安静对照组(SC组)1组、运动对照组(EC组)2组和运动补充组(ES组)8组,每组10只。SC组不运动,EC组和ES组进行一次流动水游泳池耐力游泳运动。EC组2组大鼠分别运动1.5 h和3.0 h。ES组8组大鼠,其中4组运动1.5 h,另4组运动3.0 h。SC组和EC组以5%蔗糖饮料(CHO)灌胃,ES组分别以4个肽剂量组(0.07、0.13、0.27、0.53 g/kg·bw/h)(肽/糖比分别为0.3、0.6、1.2、2.4)的肽-糖饮料(P-CHO)灌胃,灌胃频率为1次/15 min,ES组运动1.5 h和3.0 h的大鼠分别灌胃6次和12次。运动后即刻取各组大鼠血样测定19种血清游离氨基酸(FAA)、血氨(NH3)、血尿酸氮(BUN)浓度。结果:(1)与SC组比,EC组血清缬氨酸(Val)、异亮氨酸(Ile)、亮氨酸(Leu)、酪氨酸(Tyr)、苯丙氨酸(Phe)、赖氨酸(Lys)、支链氨基酸(BCAA)和芳香族氨基酸(AAA)及其比值(BCAA/AAA)、碱性氨基酸(BAA)及BUN浓度显著增加,谷氨酸(Glu)、苏氨酸(Thr)浓度、色氨酸(Trp)/支链氨基酸(BCAA)比值显著下降;(2)与SC组比,ES组Val、Ile、Leu、Tyr、Phe、Lys、BCAA、AAA、BCAA/AAA、BAA以及BUN仍显著增加,此外牛磺酸(Tau)、精氨酸(Arg)、组氨酸(His)、必需氨基酸(EAA)、含硫氨基酸(SCAA)显著增加,Glu和Trp/BCAA比值仍下降;(3)ES组与EC组比,Ala、Ile、Tyr、Thr、Lys及EAA、BAA显著增加;(4)运动3.0 h与运动1.5 h比,Val、Tau及BCAA/AAA比值显著增加,而Ala、Tyr、脯氨酸(Pro)、Arg及AAA显著降低;(5)肽补充量0.53 g/kg·bw/h与0.07、0.13、0.27 g/kg·bw/h比,血清Ala、Glu、Val、Ile、Leu、Tyr、Thr、Pro、Gly、Arg、Lys、His及EAA、BCAA、AAA、BAA显著增加,而0.07、0.13、0.27 g/kg·bw/h间的差异无显著性。(6)肽补充量与FAA的量-效分析结果显示:与EC组比,ES组运动1.5 h后,Val、Ile、Leu、Tyr、Pro、Asp、Arg、Lys、BCAA、BAA、BUN随肽补充Objective To examine the effect of supplementing beverages with different soybean peptides on serum-free amino acid(FAA)levels during 1.5-hour and 3.0-hour one-time swimming endurance exercise of rats.Method One hundred and ten Wistar rats were randomly assigned into 11 groups of 10 subjects each,including 1 sedentary control group(SC),2 exercise control groups(EC),and 8exercise supplement groups(ES).Both EC and ES groups conducted the swimming endurance exercise in a flowing water swimming pool,while SC group did not.One half of EC and ES groups exercised for 1.5 h,while the other half did 3.0 h.SC and EC groups were given 5%sucrose beverage(CHO)by gavage.ES group was gavaged with peptide-sucrose beverage(P-CHO)of 4 peptide dose groups(0.07,0.13,0.27 and 0.53 g/kg·bw/h)(peptide/carbohydrate ratio:0.3,0.6,1.2,and 2.4)every 15minutes.Blood samples were collected immediately after exercise to determine the concentration of 19FAAs,blood ammonia(NH3)and blood urine nitrogen(BUN).Results(1)Compared with SC group,a significant increase was observed in the average serum levels of valine(Val),isoleucine(Ile),leucine(Leu),tyrosine(Tyr),phenylalanine(Phe),lysine(Lys),branched-chain amino acids(BCAA),aromatic amino acids(AAA),the BCAA to AAA ratio(BCAA/AAA),basic amino acids(BAA)and BUN of EC group,but a significant decrease in the average concentrations of glutamic acid(Glu),threonine(Thr)and tryptophan(Trp)/branched-chain amino acids(BCAA)ratio.(2)Compared with SC group,the average levels of Val,Ile,Leu,Tyr,Phe,Lys,BCAA,AAA,BCAA/AAA,BAA and BUN,as well as histidine(His),essential amino acids(EAA),and sulfur-containing amino acids(SCAA)increased significantly in ES group,while the average Glu level and Trp/BCAA ratio decreased significantly.(3)Compared with EC group,the concentration of Ala,Ile,Tyr,Thr,Lys,EAA and BAA increased significantly in ES group.(4)Compared with during the 1.5 h exercise,the average levels of Val,Tau and BCAA/AAA ratio increased significantly during the 3.0 h exercise,while those of Ala,Tyr,Prolin

关 键 词：肽补充量 运动时间 血清游离氨基酸 支链氨基酸 

分 类 号：G804.2[文化科学—运动人体科学]