响应面法优化紫丁香叶总黄酮超声提取工艺  

Optimization of ultrasonic extraction process of total flavonoids from leaves of Syringa oblate by response surface methodology

作　　者：李菁 杜少兵 杨蕾 周慧慧 孟祎 薛志鹏 王小平[1,2] 白吉庆 LI Jing;DU Shaobing;YANG Lei;ZHOU Huihui;MENG Yi;XUE Zhipeng;WANG Xiaoping;BAI Jiqing(School of Pharmacy,Shaanxi University of Traditional Chinese Medicine,Xianyang 712046,China;Shaanxi Provincial Technical Service Center for Quality Monitoring of Chinese Medicine Raw Materials,Xianyang 712046,China)

机构地区：[1]陕西中医药大学药学院,陕西咸阳712046 [2]陕西省中药原料质量监测技术服务中心,陕西咸阳712046

出　　处：《广东药科大学学报》2022年第4期52-57,共6页Journal of Guangdong Pharmaceutical University

摘　　要：目的采用响应面法优化紫丁香叶中总黄酮提取工艺。方法在单因素试验的基础上,以料液比、乙醇体积分数、提取时间为影响因素,总黄酮质量分数为评价指标,确定各因素水平,采用响应曲面法优化提取工艺。结果最佳提取工艺为:料液比1∶20(g∶mL),乙醇体积分数55%,提取时间50 min,提取2次,紫丁香叶总黄酮质量分数为(106.50±1.21)mg/g,与预测值107.69 mg/g接近。结论响应面法优化的最佳工艺稳定可靠,可为紫丁香叶总黄酮高效提取提供依据。Objective To optimize the extraction process of total flavonoids from Syringa oblate leaves by response surface methodology.Methods Based on single-factor experiments,the total flavonoid content was taken as the evaluation index,the ratio of solid to liquid,the volume fraction of ethanol and the extraction time were used as the influencing factors.After the level of each factor was determined,the extraction process was optimized by response surface method.Results The optimal extraction process was as follows:the ratio of solid to liquid(g∶mL)was 1∶20,the volume fraction of ethanol was 55%,the extraction time was 50 min,and the extraction times was twice.The total flavonoid content of S.oblate leaves was(106.50±1.21)mg/g,which was close to the predicted value of 107.69mg/g.Conclusion The optimal process optimized by response surface methodology is stable and reliable,which can provide a basis for efficient extraction of total flavonoids from S.oblate leaves.

关 键 词：紫丁香叶 响应面法 总黄酮 优化工艺 

分 类 号：R282.2[医药卫生—中药学]