利用中国抗源区分强致病力大豆胞囊线虫群体的探讨  

Identification highly virulent population of soybean cyst nematode using China germplasms

作　　者：练云 魏荷 王金社 张辉 雷晨芳 李金英 卢为国 LIAN Yun;WEI He;WANG Jin-She;ZHANG Hui;LEI Chen-Fang;LI Jin-Ying;LU Wei-Guo(Henan Academy of Crop Molecular Breeding/Zhengzhou Subcenter of National Soybean Improvement Center/Key Laboratory of Oil Crops in Huanghuaihai Plains,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crops Improvement,Zhengzhou 450002,Henan,China)

机构地区：[1]河南省作物分子育种研究院/国家大豆改良中心郑州分中心/农业农村部黄淮海油料作物重点实验室/河南省油料作物遗传改良重点实验室,河南郑州450002

出　　处：《作物学报》2022年第10期2443-2450,共8页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2017YFD0101400)资助。

摘　　要：由大豆胞囊线虫(Soybean cyst nematode,Heterodera glycines,SCN)引起的病害是一种世界性大豆病害。随着强致病力大豆胞囊线虫群体X12的出现及LY1线虫群体的合成,国际通用的Riggs和HG type 2种大豆胞囊线虫生理小种鉴别模式已不能将4号生理小种、X12线虫群体、LY1线虫群体有效区分,本研究提供了一种区分这3个线虫群体的简易鉴定方法,为SCN相关研究提供技术支撑。包括以下步骤:利用感病品种在病土中繁殖备用接种的胞囊;用兴县灰皮支(ZDD2315)和PI567516C作为鉴别寄主,Lee为感病对照,接种鉴定;若兴县灰皮支和PI567516C均表现感病,表明该病土感染的是X12线虫群体;若兴县灰皮支表现抗病而PI567516C表现感病,表明该病土感染的是4号生理小种;若PI567516C表现抗病,表明该病土感染的是LY1线虫群体。以上结果表明,利用我国优异抗源兴县灰皮支和PI567516C作为鉴别寄主,能有效区分目前报道的具有强致病力的大豆胞囊线虫4号生理小种、X12和LY1线虫群体。本研究结果对筛选抗源、调查大豆胞囊线虫生理小种分布、线虫致病基因研究有重要意义。The disease caused by soybean cyst nematode(soybean cyst nematode,Heterodera glycines,SCN)is a worldwide soybean disease.The internationally used race identification models including Riggs model and Hg type for differentiate soybean cyst nematode population could not effectively distinguish the highly virulent race such as race 4,X12 population,and synthesized nematode population LY1.This study provides a simple identification method for distinguishing the three highly virulent population of the soybean cyst nematode,which would be greatly contribute to SCN research.This method included the following steps:First,we used the susceptible varieties and raised enough cysts in the soil infected highly virulent population of SCN for inoculation.Then,soybean varieties Xingxianhuipizhi(ZDD2315)and PI567516C were selected as differential hosts,and Lee was used as susceptible control for inoculation.Both ZDD2315 and PI567516C was sensitive in the inoculating reaction,indicating that the soil was infected by X12 population.If ZDD2315 was resistance while PI567516C were susceptible to the disease,indicating the soil was infected by race 4.If PI567516C was resistance to disease,it revealed that the soil was infected by LY1 population.These results indicated that the three highly virulent population including race 4,X12 population,and synthesized LY1 population could be effectively distinguished depending on the reaction of SCN on germplasms of ZDD2315 and PI567516C from China.In conclusion,these results of this study are of great significance for screening resistance germplasms,investigating the race distribution of SCN,and studying the pathogenic genes of SCN.

关 键 词：大豆胞囊线虫 生理小种 强致病力 抗源 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]