3D-printed,bi-layer,biomimetic artificial periosteum for boosting bone regeneration  

作　　者：Yage Sun Ziwei Gao Xiaoping Zhang Ziyang Xu Yahan Zhang Binbin He Rong Yang Qian Zhang Qiang Yang Wenguang Liu 

机构地区：[1]School of Material Science and Engineering,Tianjin Key Laboratory of Composite and Functional Materials,Tianjin University,Tianjin 300350,China [2]Department of Spine Surgery,Tianjin Hospital,Tianjin University,Tianjin 300211,China

出　　处：《Bio-Design and Manufacturing》2022年第3期540-555,共16页生物设计与制造（英文）

基　　金：the National Key Research and Development Program(No.2018YFA0703100);the National Natural Science Foundation of China(No.51733006).

摘　　要：Periosteum,a membrane covering the surface of the bone,plays an essential role in maintaining the function of bone tissue—and especially in providing nourishment and vascularization during the bone regeneration process.Currently,most artificial periostea have relatively weak mechanical strength and a rapid degradation rate,and they lack integrated angiogenesis and osteogenesis functions.In this study,a bi-layer,biomimetic,artificial periosteum composed of a methacrylated gelatin–nano-hydroxyapatite(GelMA-nHA)cambium layer and a poly(N-acryloyl 2-lycine)(PACG)-GelMA-Mg^(2+)fibrous layer was fabricated via 3D printing.The GelMA-nHA layer is shown to undertake the function of improving osteogenic differentiation of rat bone marrow mesenchymal stem cells with the sustainable release of Ca^(2+) from nHA nanoparticles.The hydrogen-bonding-strengthened P(ACG-GelMA-L)-Mg^(2+)hydrogel layer serves to protect the inner defect site and prolong degradation time(60 days)to match new bone regeneration.Furthermore,the released magnesium ion exhibits a prominent effect in regulating the polarization phenotype of macrophage cells into theM2 phenotype and thus promotes the angiogenesis of the human umbilical vein endothelial cells in vitro.This bi-layer artificial periosteum was implanted into a critical-sized cranial bone defect in rats,and the 12-week post-operative outcomes demonstrate optimal new bone regeneration.

关 键 词：3D printing Bi-layer periosteum scaffold Immune regulation Bone regeneration 

分 类 号：R318[医药卫生—生物医学工程]