4株内生拮抗菌对沙地云杉的促生作用  

Effects of Four Antagonistic Endophytic Fungi on the Growth on Picea mongolia

作　　者：狄佳麟 姜海燕[1] 丛林 白娜娜 张旭[1] 白慧 Di Jialin;Jiang Haiyan;Cong Lin;Bai Nana;Zhang Xu;Bai Hui(Inner Mongolia Agricultural University,Hohhot 010018,P.R.China)

机构地区：[1]内蒙古农业大学,呼和浩特010018

出　　处：《东北林业大学学报》2022年第6期39-45,共7页Journal of Northeast Forestry University

基　　金：阿鲁科尔沁旗生物多样性专项调查与保护规划服务项目(AQJYZX-2018TP047-FW);内蒙古大学生创新创业项目(201810129013)。

摘　　要：以前期筛选的4株具有强拮抗作用的沙地云杉内生真菌为试验材料,探究其促生活性,通过发酵液浸种和幼苗灌根处理,筛选出具有促生作用的内生拮抗菌。结果表明:4株内生拮抗菌对沙地云杉的种子和幼苗均表现出不同程度的促生活性。菌株SDYS63(三线镰刀菌Fusarium tricinctum)使沙地云杉种子萌发率提高25.22%,幼苗株高和地径生长提高25.24%和13.69%,总叶绿素质量分数提高71.36%,可溶性蛋白(SP)质量分数提高21.87%,丙二醛(MDA)质量摩尔浓度降低20.58%,超氧化物歧化酶(SOD)活性提高147.53%;SDYS36(互隔链格孢菌Alternaria alternata)使沙地云杉出苗率提高18.00%,根长生长提高35.68%,过氧化物酶(POD)活性提高714.29%,过氧化氢酶(CAT)活性提高69.44%;菌株SDYS95(产黄青霉菌Penicillium chrysogenum)和SDYS180(聚多曲霉菌Aspergillus sydowii)对沙地云杉种子和幼苗的促生活性较差;菌株SDYS63表现出最佳的促生活性。Four endophytic fungi of Picea mongolia with strong antagonistic effects were selected as experimental materials.The four strains of endophytic antagonists showed different degree of probiotic activity to the seeds and seedlings of P.mongolia.After inoculated with thestrain SDYS63,the seed germination rate of P.mongolica increased by 25.22%,seedling height and ground diameter growth by 25.24%and 13.69%,total chlorophyll content increased by 71.36%,SP content increased by 21.87%,MDA content decreased by 20.58%,and SOD activity increased by 147.53%.After inoculated with the strain SDYS36,the seedling emergence rate,root length,POD activity and CAT activity were increased by 18.00%,35.68%,714.29%and 69.44%compared with CK,respectively.Strain SDYS95 and SDYS180 have a weak activity to promote the life of spruce seeds and seedlings.The train SDYS63 showed the best growth promoting activity on P.mongolica.

关 键 词：沙地云杉 内生真菌 促生作用 

分 类 号：S791.18[农业科学—林木遗传育种]