二斑叶螨取食抗、感螨木薯品种对茉莉酸信号途径基因表达的影响  

Influence on Expression of Jasmonic Acid Signaling Pathway Gene in Tetranychus urticae Fed on Mite-resistant and Mite-susceptible Cassava Cultivars

作　　者：韩志玲 陈青[2,3] 梁晓 伍春玲[2,3] 刘迎 伍牧锋[2,3] 徐雪莲 HAN Zhi-ling;CHEN Qing;LIANG Xiao;WU Chun-ling;LIU Ying;WU Mu-feng;XU Xue-lian(College of Plant Protection of Hainan University,Haikou 570228;Environment and Plant Protection Institute,China Academy of Tropical Agricultural Sciences/Key Laboratory of Pests Comprehensive Governance for Tropical Crops,Ministry of Agriculture and Rural Affairs/Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests,Haikou 571101;Sanya Institute,China Academy of Tropical Agricultural Sciences/Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions,Sanya 572000)

机构地区：[1]海南大学植物保护学院,海口570228 [2]中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室/海南省热带作物病虫害生物防治工程技术研究中心,海口571101 [3]中国热带农业科学院三亚研究院/海南省南繁生物安全与分子育种重点实验室,三亚572000

出　　处：《生物技术通报》2022年第6期211-220,共10页Biotechnology Bulletin

基　　金：国家重点研发计划专项(2020YFD1000603);国家木薯产业技术体系虫害防控岗位科学家专项(CARS-11-HNCQ);农业农村部农业资源调查与保护利用专项(NFZX-2021);海南省重大科技计划项目(ZDKJ202002)。

摘　　要：明确二斑叶螨取食影响木薯茉莉酸信号途径基因表达的害螨密度和取食时间效应,为阐明茉莉酸信号途径在木薯抗虫性中的重要作用及抗螨种质资源鉴定评价提供理论依据及参考指标。以抗螨木薯品种C1115和感螨木薯品种面包为材料,分析与植物防御相关的茉莉酸号信号途径基因(DAD1、LOX2、OPR3和JAR1)在二斑叶螨以不同虫口密度(15、25、35、45、50和55头螨/叶)取食不同时间(1、2、4和8 d)后的表达量变化情况。结果表明,以45-50头螨/叶的害螨密度分别取食抗、感螨木薯品种2 d后,LOX2、OPR3和JAR1的表达量均较螨害前显著提高,并且上述3个基因在抗螨木薯品种中的表达量显著高于感螨木薯品种,而DAD1的表达量随害螨密度的提高和取食时间的增加波动较大。在较高的害螨密度和较短的为害时间处理时,茉莉酸信号途径基因LOX2、OPR3和JAR1在抗螨木薯品种中的表达量显著高于感螨木薯品种,LOX2、OPR3和JAR1可能与木薯对二斑叶螨的抗性有关。This study aims to clarify how the mite density and feeding time affect gene expressions of jasmonic acid signaling pathway when Tetranychus urticae fed on mite-resistant and mite-susceptible cassava cultivars,which can not only provide a theoretical basis for elucidating the important role of jasmonic acid signal pathway in cassava resistance to insect,but also can offer references for identifying and evaluating mite resistant germplasm resources.Here,mites with different densities(15,25,35,45,50 and 55 mites/leaf)were inoculated on the leaves of mite-resistant cassava cultivar C1115 and mite-susceptible cassava cultivar Mianbao,respectively.And then the expressions of jasmonic acid signaling pathway genes(DAD1,LOX2,OPR3 and JAR1)were analyzed at 1,2,4 and 8 d post-infestation,respectively.The results showed that the expressions of LOX2,OPR3 and JAR1 in 2 d post-infestation either mite-resistant cassava C1115 and mite-susceptible cassava Mianbao significantly increased compared with those before infested,in addition,the expressions of those three genes were significantly higher in C1115 than Mianbao(P<0.05),while the expressions of DAD1 showed great fluctuation as the mite density and infestation time increased.This study revealed that under the conditions of relative high mite density and short mite infestation period,the expressions of jasmonic acid signaling pathway genes like LOX2,OPR3 and JAR1 were significantly higher in mite-resistant cassava cultivars compared with the susceptible ones.Those genes might probably correlate with cassava resistance to mite,which needs further investigation.

关 键 词：二斑叶螨 抗、感木薯品种 害螨密度 取食时间 茉莉酸途径 基因表达 

分 类 号：S435.33[农业科学—农业昆虫与害虫防治]