舒芬太尼后处理对大鼠缺血再灌注损伤后心肌自噬的影响  

The effect of sufentanil postconditioning on the cell autophagy after myocardial ischemia-reperfusion injury in rats

作　　者：徐达[1] 许旭东[1] 潘春英[1] 郦惠芳[1] 商建飞[1] 张爱萍[1] Xu Da;Xu Xudong;Pan Chunying;Li Huifang;Shang Jianfei;Zhang Aiping(Department of Anesthesiology,Changzhou Hospital of Traditional Chinese Medicine,Changzhou 213000,China)

机构地区：[1]江苏省常州市中医医院麻醉科,213000

出　　处：《心脑血管病防治》2022年第2期27-31,F0003,共6页CARDIO-CEREBROVASCULAR DISEASE PREVENTION AND TREATMENT

摘　　要：目的 探讨舒芬太尼后处理对心肌缺血再灌注损伤(MIRI)大鼠心肌细胞自噬作用的影响,以及高迁移率族蛋白B1(HMGB1)/Toll样受体4(TLR4)/核因子κB(NF-κB)信号通路的调节机制。方法 将大鼠随机分为:假手术组、模型组、观察组、对照组,采用结扎左管状动脉前降支构建缺血再灌注大鼠模型,假手术组只穿线不结扎,观察组、对照组大鼠在再灌注结束10 min股静脉注射1μg/kg的舒芬太尼,对照组股静脉注射2 mg/kg的HMGB1特异性抗体,假手术组、模型组大鼠注射等剂量的0.9%氯化钠溶液。TTC检测各组大鼠心肌梗死面积比例;免疫组化检测大鼠心肌细胞中自噬标志物Beclin1的表达;Western blot检测各组大鼠心肌组织中HMGB1、TLR4、NF-κB的表达水平。结果 假手术组、模型组、观察组、对照组大鼠心肌梗死面积比例、Beclin1阳性细胞比例、HMGB1、TLR4、NF-κB的表达差异均具有统计学意义(F=33.764、43.257、6.831、5.093、5.672,均P <0.05),与假手术组相比,模型组、观察组、对照组大鼠心肌梗死面积比例、Beclin1阳性细胞比例、HMGB1、TLR4、NF-κB的表达明显升高(均P <0.05);与模型组相比,观察组、对照组大鼠心肌梗死面积、Beclin1阳性细胞比例、HMGB1、TLR4、NF-κB的表达明显降低(均P <0.05)。结论 舒芬太尼后处理能明显抑制MIRI后大鼠的心肌自噬,降低细胞的凋亡率和心肌梗死面积,缓解心肌损伤,这可能与下调HMGB1/TLR4/NF-κB信号的表达有关。Objective To explore the effect of sufentanil postconditioning on the cell autophagy after myocardial ischemia-reperfusion injury in rats, and the regulation mechanism of high mobil ity group protein B1(HMGB1)/toll-like receptor 4(TLR4)/nuclear factor κB(NF-κB) signaling pathway. Methods Rats were randomly divided into sham operation group, model group, observation group and control group. The anterior descending branch of the left tubular artery was ligated to construct an ischemia-reperfusion rat model. The sham operation group only had threading without ligation. The rats in the observation group and the control group were injected with 1 μg/kg sufentanil in the femoral vein 10 minutes after reperfusion, the control group was injected with 2 mg/kg HMGB1 specific antibody in the femoral vein, and the rats in the sham operation group and model group were injected with the same dose 0.9% sodium chloride solution. TTC staining was used to detect the area of myocardial infarction. Immunohistochemistry was used to detect the expression of autophagy marker Beclin1.Western blot was used to detect the expression levels of HMGB1, TLR4 and NF-κB. Results The differences on the ratio of myocardial infarction area, the proportion of Beclin1 positive cells, the expression of HMGB1, TLR4, and NF-κB were statistically significant among the sham operation group, model group, observation group and control group(F=33.764, 43.257, 6.831, 5.093, 5.672;all P < 0.05). Compared with the sham operation group, the ratio of myocardial infarction area, the proportion of Beclin1 positive cells, the expression of HMGB1, TLR4, and NF-κB in the model group, observation group and control group significantly increased(all P < 0.05). Compared with the model group, the ratio of myocardial infarction area, the proportion of Beclin1 positive cells, the expression of HMGB1, TLR4, and NF-κB in the observation group and control group significantly decreased(all P < 0.05). Conclusion Sufentanil postconditioning can significantly inhibit myocard

关 键 词：舒芬太尼后处理 心肌缺血再灌注损伤 细胞自噬 高迁移率族蛋白B1/Toll样受体4/核因子κB信号通路 

分 类 号：R542.22[医药卫生—心血管疾病]