陕北黄土丘陵沟壑区苔藓内生细菌群落多样性分析  

Diversity Endophytic Bacterial Community of Mosses in Hilly and Gully Region of Loess Plateau of Northern Shaanxi

作　　者：邓振山[1] 薛军艳 郭旻皓 柳晓东 姜影影 贺晓龙[1] 陈浩 DENG Zhenshan;XUE Junyan;GUO Minhao;LIU Xiaodong;JIANG Yingying;HE Xiaolong;CHEN Hao(College of Life Science,Yan’an University,Yan’an Shaanxi 716000,China;College of life Science,Northwest University,Xi’an 710069,China)

机构地区：[1]延安大学生命科学学院,陕西延安716000 [2]西北大学生命科学学院,西安710069

出　　处：《西北农业学报》2022年第7期902-912,共11页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省科技统筹创新工程项目(2016TTC-N-3-1);陕西省县域重点科技项目(2018XY-14);陕西省科技惠民计划项目(2014HM-14);延安市科技局重大专项项目(2014CGZH-06);延安市菌草工程研究中心专项基金;延安市生物资源开发与利用科技创新团队专项基金;2019年陕西省农业厅农业绿色技术研发集成项目;2019年第八批陕西省省级农业标准化示范区项目。

摘　　要：以延安市当地藓结皮中的土著优势种土生对齿藓(Didymodon vinealis)、反扭藓(Timmiella anomala)和青藓(Brachythecium albicans)为研究对象,分析3种苔藓植株内生细菌的群落组成和多样性,揭示3种不同品种的苔藓植株内生细菌的群落结构及多样性差异。采用高通量测序技术分析3种苔藓植株内生细菌16S rRNA基因V3~V4变异区序列,使用生物信息学方法分析3种苔藓内生细菌的群落结构及多样性。结果表明:土生对齿藓、反扭藓、青藓3种苔藓测序共获得178173条有效序列,在97%一致性下共产生705个有效的OTUs。在门、纲和属水平上,土生对齿藓的优势类群分别是蓝藻细菌门(Cyanobacteria,57.91%)、变形菌门(Proteobacteria,41.00%),γ-变形菌纲(Gammaproteobacteria,35.02%)、α-变形菌纲(Alphaproteobacteria,5.92%)和假单胞细菌属(Pseudomonas,24.92%)、(Massilia,6.26%);反扭藓分别为蓝藻细菌门(Cyanobacteria,40.05%)、变形菌门(Proteobacteria,40.88%),α-变形菌纲(Alphaproteobacteria,30.87%)和(Phytohabitans,4.10%)、鞘氨醇单胞菌属(Sphingomonas,3.93%);青藓分别是蓝藻细菌门(Cyanobacteria,80.49%)、变形菌门(Proteobacteria,15.26%),γ-变形菌纲(Gammaproteobacteria,7.66%)、α-变形菌纲(Alphaproteobacteria,7.45%)和假单胞细菌属(Pseudomonas,6.46%)。Alpha多样性和Beta多样性分析表明,反扭藓的Chao1指数(370.667)和Shannon指数(5.368)最大,基于韦恩图分析3种苔藓存在87个共有OTUs,土生对齿藓、反扭藓和青藓独有OTUs分别为7、175和13,说明3种苔藓不仅有较高的细菌多样性,还存在一定的差异。典范对应分析(Canonical Correlation Analysis,CCA)结果表明,土壤中有效氮(AN)和有机质(OM)的含量是影响3种苔藓内生细菌的群落多样性变化的主要因素。3个不同品种的苔藓的内生细菌种类丰富,群落结构组成和丰度均存在较大的差异,且苔藓所生长的微环境如有效氮和有机质的含量会对其内生细菌群落结构�Endophytic bacteria might be beneficial for plant growth and yield,however,the composition of the endophytic bacterial community associated to moss is poorly characterized.As indigenous dominant species of moss crusts,Didymodon vinealis,Timmiella anomala and Brachythecium albicans were chosen as object to study the community structure and endophytic bacteria diversity by using Illumina HiSeq second-generation high-throughput sequencing technology in hilly region of loess plateau of northern Shaanxi.The variation region of 16s rRNA geneV3-V4 of three species associated with mosses was sequenced by using high-throughput next-generation sequencing,and the community structure and diversity of endophytic bacteria of three mosses were analyzed by bioinformatics analysis.A total of 178173 effective sequences were obtained by sequencing three mosses of Didymodon vinealis,Timmiella anomala and Brachythecium albicans,and a total of 705 effective OTUs were generated at a similarity level of 97%.The dominant phylum and genus of Didymodon vinealis were Cyanobacteria(57.91%),Proteobacteria(41.00%),Gammaproteobacteria(35.02%),Alphaproteobacteria(5.92%)and Pseudomonas(24.92%),Massilia(6.26%);The dominant phylum and genus of Timmiella anomala were Cyanobacteria(40.05%),Proteobacteria(40.88%),Alphaproteobacteria(30.87%)and Phytohabitans(4.10%),Sphingomonas(3.93%).The dominant phylum,class and genus of Brachythecium albicans were Cyanobacteria(80.49%),Proteobacteria(15.26%),Gamma-proteobacteria(7.66%),Alphaproteobacteria(7.45%)and Pseudomonas(6.46%).The Chao1 index(370.667)and Shannon index(5.368)of Timmiella anomala were the largest byα-diversity analysis.Based on Venn diagram analysis of OTUs,there were 87 OTUs,and the unique OTUs of Didymodon vinealis and Brachythecium albicans were respectively 7,175 and 13,which indicated that the three mosses not only had high bacterial diversity,but also had some differences.The results of canonical correlation analysis(CCA)showed that the contents of available nitrogen(AN)and organic matte

关 键 词：高通量测序 藓结皮 苔藓植物 内生细菌 群落多样性 

分 类 号：Q949.2[生物学—植物学]