蛋白核小球藻的培养及应用研究  

Cultivation and Application of Chlorella pyrenoidosa

作　　者：岳海艳 刘小杰 孙敏 李娜 刘占峰 YUE Hai-yan;LIU Xiao-jie;SUN Min;LI Na;LIU Zhan-feng(Shanghai Urban Construction Vocational College,Shanghai 201415;Shanghai Research Institute of Chemical Industry Co.Ltd,Shanghai 200062)

机构地区：[1]上海城建职业学院,上海201415 [2]上海化工研究院有限公司,上海200062

出　　处：《粮油食品科技》2022年第4期178-184,共7页Science and Technology of Cereals,Oils and Foods

基　　金：上海城建职业学院教育科学研究项目(cjky202106)。

摘　　要：选用密闭气升式反应器进行自养培养蛋白核小球藻,通过单因素优化获得碳源二氧化碳的通气量2%,氮源硝酸钠为10 g/L;通过对蛋白核小球藻培养基的均匀设计,获得了适宜的培养基组成:磷酸氢二钾0.6 g/L,硫酸镁0.2 g/L,硝酸钠11 g/L,硫酸钾0.8 g/L,柠檬酸铁胺0.006 g/L,氯化钙0.05 g/L,维生素B10.11 g/L,EDTANa20.01 g/L,钨酸钠0.08×10^(–3 )g/L,氯化镍0.02×10^(–3) g/L;实验采用中间白光四周彩光的合适光源,用除氧剂使氧气维持在20%左右,获得了8.648 g/L的干菌体。经分析,蛋白核小球藻蛋白质含量高达58 g/100 g,同时含有丰富的氨基酸和矿物质元素。用蛋白核小球藻替代艾草粉,制作出了蛋白核小球藻青团。A closed gas-up reactor was selected for self-cultured Chlorella pyrenoidosa,the ventilation of carbon dioxide from carbon source obtained by univariate optimization was 2%,and the sodium nitrate of nitrogen source was 10 g/L.More suitable medium components were obtained through the uniform design of Chlorella pyrenoidosa culture medium:K_(2)HPO_(4) of 0.6 g/L,MgSO_(4) of 0.2 g/L,NaNO_(3) of 11 g/L,K_(2)SO_(4) of 0.8 g/L,ammonium ferric citrate of 0.006 g/L,CaCl_(2) of 0.05 g/L,vitaminB1 of 0.11 g/L,EDTANa_(2) of 0.01 g/L,Na_(2)WO_(4) of 0.08×10^(–3) g/L,NiCl_(2) of 0.02×10^(–3) g/L;In the experiment,a suitable light source with intermediate white light and surrounding colored light was used,and the oxygen was maintained at about 20%with an oxygen scavenger,under which 8.648 g/L dry cell was obtained finally.The protein content of Chlorella pyrenoidosa is as high as 58 g/100 g,and the amino acid and mineral elements of Chlorella pyrenoidosa is also rich by analysis.We studied the replacement of wormwood powder with Chlorella pyrenoidosa,and produced Chlorella pyrenoidosa qingtuan.

关 键 词：气升式反应器 蛋白核小球藻 培养 青团 

分 类 号：Q815[生物学—生物工程] TS213.2[轻工技术与工程—粮食、油脂及植物蛋白工程]