动物源肠球菌对饲用抗菌促生长剂流行病学临界值的建立  

Establishment of the Epidemiological Cut-off Values of Enterococcus Isolated from Animals for Antimicrobial Growth Promoters

作　　者：杨钰莹 王少林[1] YANG Yuying;WANG Shaolin(College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510642,China)

机构地区：[1]中国农业大学动物医学院,北京100193 [2]岭南现代农业科学与技术广东省实验室,广州510642

出　　处：《中国畜牧兽医》2022年第7期2788-2795,共8页China Animal Husbandry & Veterinary Medicine

基　　金：农业农村部动物源性耐药性监测计划。

摘　　要：【目的】建立动物源肠球菌对抗菌促生长剂的流行病学临界值,并基于此了解2019-2021年动物源肠球菌对饲用抗菌促生长剂的耐药情况。【方法】测定2019-2021年从北京、河北、四川、山西、陕西和内蒙古养殖场动物样本中分离得到的肠球菌对7种抗菌促生长剂(吉他霉素、黄霉素、恩拉霉素、那西肽、阿维拉霉素、维吉尼亚霉素和杆菌肽)的最小抑菌浓度(MIC)值。根据CLSI-VET中规定的方法,利用统计软件ECOFFinder对MIC分布数据进行非线性回归模拟,以95%置信区间下的流行病学临界值计算动物源肠球菌对7种抗菌促生长剂的耐药率。【结果】确定了动物源肠球菌对吉他霉素、黄霉素、恩拉霉素、那西肽、阿维拉霉素、维吉尼亚霉素和杆菌肽的流行病学临界值分别为8、8、8、0.25、8、8和32μg/mL。由此计算的药物耐药率为64.38%、34.48%、80.06%、14.69%、19.06%、50.69%和24.96%,其中动物源肠球菌对恩拉霉素、吉他霉素和维吉尼亚霉素的耐药情况最为严重,而对那西肽最敏感。在2019-2021年的耐药率变化情况统计中,黄霉素和杆菌肽的耐药率下降趋势较为明显。【结论】本研究制定了动物源肠球菌对7种养殖业常用抗菌促生长剂的流行病学临界值,可作为动物源肠球菌对饲用抗菌促生长剂敏感和耐药的初步判定标准,为进一步制定耐药折点,监测动物源肠球菌对抗菌促生长剂的耐药情况变化提供数据基础和科学依据。【Objective】This study was aimed to establish the epidemiological cut-off values of Enterococcus isolated from animals for antimicrobial growth promoters and further to understand the resistance rate of animal-derived Enterococcus to antimicrobial growth promoters from 2019 to 2021.【Method】The MICs of Enterococcus isolated from breeding farms in Beijing,Hebei,Sichuan,Shanxi,Shaanxi and Inner Mongolia from 2019 to 2021 to seven antimicrobial growth promoters(kitasamycin,flavomycin,enramycin,nasipeptide,averamycin,viginiamycin and bacitracin)were tested.According to the method specified in CLSI-VET,the non-linear regression simulation of MIC distribution data was carried out using the statistical tool ECOFFinder.The drug resistance rate of Enterococcus from animals to 7 kinds of antibacterial growth promoters was calculated by the epidemiological cut-off values under 95%confidence interval.【Result】The epidemiological cut-off values of kitasamycin,flavomycin,enramycin,narceptide,averamycin,virinomycin and bacitracin were 8,8,8,0.25,8,8 and 32μg/mL,respectively.Based on this,the drug resistance rates calculated were 64.38%,34.48%,80.06%,14.69%,19.06%,50.69%and 24.96%,respectively.Among them,Enterococcus was most resistant to enramycin,kitasamycin and virginiamycin,but most sensitive to nazeptide.In the statistics from 2019 to 2021,the drug resistance rates of flavomycin and bacitracin decreased relatively obviously.【Conclusion】This study established the epidemiological cut-off values of animal-derived Enterococcus against seven antimicrobial growth promoters commonly used in aquaculture.It could be used as a preliminary criterion for determining the sensitivity and resistance of animal-derived Enterococcus to antimicrobial growth promoters and provide a scientific basis for further establishing drug resistance points and monitoring the changes of drug resistance of animal-derived Enterococcus to antimicrobial growth promoters.

关 键 词：肠球菌 抗菌促生长剂 流行病学临界值 耐药性 

分 类 号：S852.611[农业科学—基础兽医学]