Cerebrovascularβ-dystroglycan immunoreactivity in vertebrates:not detected in anurans and in the teleosts Ostariophysi and Euteleostei  

作　　者：Mihály KÁLMÁN David L.LŐRINCZ Olivér M.SEBŐK Csilla ARI Erzsébet OSZWALD Hiroaki SOMIYA Veronika JANCSIK 

机构地区：[1]Department of Anatomy,Histology and Embryology,Semmelweis University,Budapest,Hungary [2]University of Leicester,Dept.of Neuroscience,Psychology and Behaviour,Leicester,United Kingdom [3]Hyperbaric Neuroscience Research Lab.,Dept of Psychology,University of South Florida,Tampa,Florida,USA [4]Department of Environmental Biology,Chubu University,Chubu,Japan [5]Department of Anatomy,Veterinary University,Budapest,Hungary

出　　处：《Integrative Zoology》2020年第1期16-31,共16页整合动物学（英文版）

摘　　要：The aim of the present paper was to check for the presence of cerebrovascular dystroglycan in vertebrates,because dystroglycan,which is localized in the vascular astroglial end-feet,has a pivotal function in glio-vascular connections.In mammalian brains,the immunoreactivity ofβ-dystroglycan subunit delineates the vessels.The results of the present study demonstrate similar patterns in other vertebrates,except for anurans and the teleost groups Ostariophysi and Euteleostei.In this study,we investigated 1 or 2 representative species of the main groups of Chondrichthyes,teleost and non-teleost ray-finned fishes,urodeles,anurans,and reptiles.We also investigated 5 mammalian and 3 bird species.Animals were obtained from breeders or fishermen.The presence ofβ-dystroglycan was investigated immunohistochemically in free-floating sections.Pre-embedding electron microscopical immunohistochemistry on Heterodontus japonicus shark brains demonstrated that in Elasmobranchii,β-dystroglycan is also localized in the perivascular glial end-feet despite the different construction of their blood-brain barrier.The results indicated that the cerebrovascularβ-dystroglycan immunoreactivity disappeared separately in anurans,and in teleosts,in the latter group before its division to Ostariophysi and Euteleostei.Immunohistochemistry in muscles and western blots from brain homogenates,however,detected the presence ofβ-dystroglycan,even in anurans and all teleosts.A possible explanation is that in the glial end-feet,β-dystroglycan is masked in these animals,or disappeared during adaptation to the freshwater habitat.

关 键 词：ANURANS CEREBROVASCULAR DYSTROGLYCAN ELASMOBRANCHS TELEOSTS 

分 类 号：Q95[生物学—动物学]