应用同位素标记相对和绝对定量蛋白质组学方法筛选浆细胞性乳腺炎病人差异表达蛋白质  

Application of isobaric tags for relative and absolute quantitation quantitative proteomics in application of differentially expressed proteins for plasma cell mastitis

作　　者：陈波[1] 赵侃侃 卢文亮[1] 郑媛[1] CHEN Bo;ZHAO Kankan;LU Wenliang(Department of Thyroid and Breast Surgery,Hubei Maternal and Child Health Hospital,Hubei Province Key Laboratory of Occupational Hazard Identification and Control,Medical College,Wuhan university od Science and Technology,Wuhan 430070,China)

机构地区：[1]湖北省妇幼保健院甲状腺乳腺外科,武汉科技大学职业危害识别与控制湖北省重点实验室,430070

出　　处：《临床外科杂志》2022年第5期463-466,共4页Journal of Clinical Surgery

基　　金：湖北省卫生健康科研基金资助项目(WJ2019H191)。

摘　　要：目的分析浆细胞性乳腺炎组织的蛋白表达谱,寻找浆细胞性乳腺炎的关键分子。方法选择4例浆细胞性乳腺炎病人,术中留取病灶组织、正常组织标本,提取蛋白,采用同位素标记相对和绝对定量蛋白质组学技术分析在浆细胞性乳腺炎中差异表达的蛋白质,并进行功能富集分析。结果在浆细胞性乳腺炎中,鉴定出915个差异蛋白(上调795个蛋白,下调120个蛋白)。功能富集分析表明这些差异蛋白主要参与细胞黏附、炎症反应等。KEGG通路分析发现,这些差异蛋白主要参与免疫系统相关信号通路。结论浆细胞乳腺炎中差异表达的蛋白质,参与了相关信号通路,可能是浆细胞性乳腺炎发生发展的关键分子。Objective To explore the protein expression profile and key protein for plasma cell mastitis by proteomics techniques.Methods We collected the plasma cell mastitis tissues and adjacent normal tissues following surgical removal of affected tissues from 4 plasma cell mastitis patients.Then,we extracted the proteins,analyzed the differentially expressed proteins in plasma cell mastitis by applying isobaric tags for relative and absolute quantitation(iTRAQ)technology,and conducted functional enrichment analysis using these differentially expressed proteins.Results In our study,915 differentially expressed proteins were identified including 795 upregulated proteins and 120 downregulated proteins in plasma cell mastitis tissues.Functional enrichment analysis showed these differentially expressed proteins were mainly involved in cell adhesion,and inflammatory response.KEGG pathway analysis showed these differentially expressed proteins were mainly enriched in immune system associated pathway.Conclusion Differentially expressed proteins in plasma cell mastitis are involved in related signaling pathways and may be key molecules in the development of plasma cell mastitis.

关 键 词：浆细胞性乳腺炎 同位素标记相对和绝对定量 蛋白质组学 分子标记物 

分 类 号：R655.8[医药卫生—外科学]