芳香烃受体mRNA、白细胞介素22在2型糖尿病中的作用研究  

Roles of aryl hydrocarbon receptor mRNA and interleukin-22 in type 2 diabetes mellitus

作　　者：刘艳秋[1] 范海迪 孙健 薛莲 孟月 林宁[2] LIU Yanqiu;FAN Haidi;SUN Jian;XUE Lian;MENG Yue;LIN Ning(Department of Clinical Laboratory,Branch Hospital of Huai'an City First People's Hospital in Jiangsu Province,Huai'an,Jiangsu,223002;Department of Clinical Laboratory,Huai'an First Hospital Affiliated to Nanjing Medical University,Huai'an,Jiangsu,223300)

机构地区：[1]江苏省淮安市第一人民医院分院检验科,江苏淮安223002 [2]南京医科大学附属淮安第一医院检验科,江苏淮安223300

出　　处：《实用临床医药杂志》2022年第13期30-34,共5页Journal of Clinical Medicine in Practice

基　　金：江苏省淮安市自然科学研究计划资助项目(HAB202137)。

摘　　要：目的探讨2型糖尿病(T2DM)患者外周血单个核细胞中芳香烃受体(AhR)mRNA表达以及血清白细胞介素22(IL-22)水平。方法将49例糖尿病前期患者、48例T2DM患者和50例健康对照者分别纳入糖尿病前期组、T2DM组、对照组。采用逆转录聚合酶链式反应(RT-PCR)法测定外周血单个核细胞中AhR mRNA的表达;采用酶联免疫吸附法(ELISA)检测各组血清IL-22水平。测定各组空腹血糖(FBG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、胆固醇(CHO)、甘油三酯(TG)、空腹胰岛素(FINS)、餐后血糖(PPG)、糖化血红蛋白(HbA1c)和超敏C反应蛋白(hs-CRP)。结果糖尿病前期组、T2DM组FBG、TG、PPG、HbA1c、hs-CRP均高于对照组,T2DM组HDL-C低于对照组,差异有统计学意义(P<0.01);T2DM组FBG、TG、PPG、HbA1c高于糖尿病前期组,HDL-C低于糖尿病前期组,差异有统计学意义(P<0.05或P<0.01)。糖尿病前期组FINS、血清IL-22高于对照组,差异有统计学意义(P<0.01);T2DM组FINS、血清IL-22、AhR mRNA相对表达含量均高于对照组和糖尿病前期组,差异有统计学意义(P<0.01)。Pearson相关性分析显示,AhR mRNA、IL-22与年龄、CHO、TG、LDL-C、体质量指数均无相关性(P>0.05),与FBG、FINS、PPG、HbA1c、hs-CRP呈正相关(P<0.05或P<0.01)。结论通过检测AhR mRNA、IL-22水平对T2DM患者进行病情评估和预后判断具有一定的临床意义,AhR mRNA、IL-22或可成为糖尿病治疗研究的新靶标。Objective To investigate the expression of aryl hydrocarbon receptor(AhR)mRNA in peripheral blood mononuclear cells and serum level of interleukin-22(IL-22)in patients with type 2 diabetes mellitus(T2DM).Methods A total of 49 patients with pre-diabetes mellitus,48 patients with T2DM and 50 healthy controls were respectively included in pre-diabetes group,T2DM group and control group.The expression of AhR mRNA in peripheral blood mononuclear cells was determined by reverse transcription polymerase chain reaction(RT-PCR);serum IL-22 level was detected by enzyme-linked immunosorbent assay(ELISA)in each group.Fasting blood glucose(FBG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),cholesterol(CHO),triglyceride(TG),fasting insulin(FINS),postprandial glucose(PPG),glycosylatedhemoglobin(HbA1c)and hypersensitive C-reactive protein(hs-CRP)were measured in each group.Results Levels of FBG,TG,PPG,HbA1c and hs-CRP in the pre-diabetes group and the T2DM group were significantly higher than those in the control group,and HDL-C in the T2DM group was significantly lower than that in the control group(P<0.01);levels of FBG,TG,PPG and HbA1c in the T2DM group were significantly higher than those in the pre-diabetes group,while the HDL-C level was significantly lower than that in the pre-diabetes group(P<0.05 or P<0.01).FINS and serum IL-22 in the pre-diabetes group were significantly higher than those in the control group(P<0.01);the FINS,serum IL-22 and relative expression of AhR mRNA in the T2DM group were significantly higher than those in the control group and the pre-diabetes group(P<0.01).Pearson correlation analysis showed that AhR mRNA and IL-22 had no correlations with age,CHO,TG,LDL-C and body mass index(P>0.05),but were positively correlated with FBG,FINS,PPG,HbA1c and hs-CRP(P<0.05 or P<0.01).Conclusion Detection of AhR mRNA and IL-22 level have certain clinical significance in evaluating the disease condition and prognosis of T2DM patients,which indicate AhR mRNA and IL-22 may b

关 键 词：2型糖尿病 芳香烃受体 白细胞介素22 空腹血糖 空腹胰岛素 糖化血红蛋白 

分 类 号：R587.1[医药卫生—内分泌] R392.6[医药卫生—内科学]