A silent two-photon imaging system for studying in vivo auditory neuronal functions  

作　　者：Xindong Song Yueqi Guo Chenggang Chen Xiaoqin Wang 

机构地区：[1]Laboratory of Auditory Neurophysiology,Department of Biomedical Engineering,Johns Hopkins University School of Medicine,Baltimore,MD 21205,USA

出　　处：《Light(Science & Applications)》2022年第5期888-900,共13页光（科学与应用)（英文版）

基　　金：supported by the National Institutes of Health grants#DC003180,DC005808;supported by a fellowship from the Kavli Neuroscience Discovery Institute at JHU.

摘　　要：Two-photon laser-scanning microscopy has become an essential tool for imaging neuronal functions in vivo and has been applied to different parts of the neural system,including the auditory system.However,many components of a two-photon microscope,such as galvanometer-based laser scanners,generate mechanical vibrations and thus acoustic artifacts,making it difficult to interpret auditory responses from recorded neurons.Here,we report the development of a silent two-photon imaging system and its applications in the common marmoset(Callithrix Jacchus),a non-human primate species sharing a similar hearing range with humans.By utilizing an orthogonal pair of acoustooptical deflectors(AODs),full-frame raster scanning at video rate was achieved without introducing mechanical vibrations.Imaging depth can be optically controlled by adjusting the chirping speed on the AODs without any mechanical motion along the Z-axis.Furthermore,all other sound-generating components of the system were acoustically isolated,leaving the noise floor of the working system below the marmoset’s hearing threshold.Imaging with the system in awake marmosets revealed many auditory cortex neurons that exhibited maximal responses at low sound levels,which were not possible to study using traditional two-photon imaging systems.This is the first demonstration of a silent two-photon imaging system that is capable of imaging auditory neuronal functions in vivo without acoustic artifacts.This capacity opens new opportunities for a better understanding of auditory functions in the brain and helps isolate animal behavior from microscope-generated acoustic interference.

关 键 词：system utilizing GENERATING 

分 类 号：TH742[机械工程—光学工程] R338[机械工程—仪器科学与技术]