两种氟苯尼考注射液在鸡体内比较药动学研究  

Comparative Pharmacokinetic of Two Florfenicol Injections in Chicken

作　　者：杨洁 李慧素 彭丽 孟蕾 张盼盼 吴宁鹏 YANG Jie;LI Hui-su;PENG Li;MENG Lei;ZHANG Pan-pan;WU Ning-peng(Henan Institute of Veterinary Drug and Feed Control, Zhengzhou 450008,China)

机构地区：[1]河南省兽药饲料监察所,郑州450008

出　　处：《中国兽药杂志》2022年第6期15-20,共6页Chinese Journal of Veterinary Drug

摘　　要：为比较两种氟苯尼考注射液的药物代谢动力学,选择30只健康鸡随机分为两组,分别单剂量20 mg/kg bw肌内注射受试制剂和参比制剂,于给药后0.167、0.33、0.5、0.75、1、1.5、2、4、6、8、12、24、48 h翼下静脉采集血样。用超高效液相色谱法(UPLC-UV)测定血浆中氟苯尼考的含量,并用WinNonlin 8.1非房室模型计算主要药代动力学参数。结果显示,受试制剂和参比制剂的t_(1/2)分别为(3.39±2.65)和(4.47±3.14)h,T_(max)分别为(0.66±0.30)和(0.77±0.30)h,C_(max)分别为(7.06±2.35)和(8.24±4.54)μg/mL,AUC_(0→t)分别为(19.05±5.79)和(21.76±6.71)(μg/mL)h,AUC_(0→∞)分别为(20.11±6.36)和(23.04±6.91)(μg/mL)h,MRT分别为(3.25±1.25)和(3.55±0.96)h,相对生物利用度为87.55%。结果表明,二者主要药动学参数无显著性差异(P>0.05),但受试制剂相对生物利用度较低。In order to compare the pharmacokinetics of two kinds of florfenicol injections,30 healthy chickens were randomly divided into two groups,and were given respectively a single dose of test preparation or reference preparation by intramuscular injection(20 mg/kg bw).Blood samples were collected from the inferior wing vein at different times(0.167,0.33,0.5,0.75,1,1.5,2,4,6,8,12,24,48 h).Plasma concentrations of florfenicol were measured by UPLC-UV,and the pharmacokinetic parameters were calculated by WinNonlin 8.1 software.The main pharmacokinetic parameters of the test preparation and reference preparation were as follows:t_(1/2) were(3.39±2.65)and(4.47±3.14)h,T_(max) were(0.66±0.30)and(0.77±0.30)h;C_(max) were(7.06±2.35)and(8.24±4.54)μg/mL;AUC_(0→t) were(19.05±5.79)and(21.76±6.71)(μg/mL)h;AUC_(0→∞) were(20.11±6.36)and(23.04±6.91)(μg/mL)h;MRT were(3.25±1.25)and(3.55±0.96)h,the relative bioavailability was 87.55%.The results showed that there was no significant difference in pharmacokinetic parameters between the two preparation(P>0.05),the relative bioavailability of the tested preparation is low.

关 键 词：氟苯尼考 鸡 超高效液相色谱法 比较药动学 

分 类 号：S859.796[农业科学—临床兽医学]