低温促进表面等离激元共振效应及肌酐的超灵敏表面增强拉曼散射探测  

Low temperature-promoted surface plasmon resonance effect and ultrasensitive surface-enhanced Raman scattering detection of creatinine

作　　者：厉桂华[1] 张梦雅 马慧 田悦 焦安欣 郑林启 王畅 陈明[2] 刘向东[2] 李爽[4] 崔清强[2] 李冠华 Li Gui-Hua;Zhang Meng-Ya;Ma Hui;Tian Yue;Jiao An-Xin;Zheng Lin-Qi;Wang Chang;Chen Ming;Liu Xiang-Dong;Li Shuang;Cui Qing-Qiang;Li Guan-Hua(School of Information Science and Engineering,Shandong Agricultural University,Tai’an 271018,China;School of Physics,Shandong University,Jinan 250100,China;Department of Pulmonary and Critical Care Medicine,Jinan Central Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250100,China;College of Science,Shandong Jianzhu University,Jinan 250100,China)

机构地区：[1]山东农业大学信息科学与工程学院,泰安271018 [2]山东大学物理学院,济南250100 [3]山东大学齐鲁医学院济南市中心医院,呼吸与危重症医学科,济南250100 [4]山东建筑大学理学院,济南250100

出　　处：《物理学报》2022年第14期182-191,共10页Acta Physica Sinica

基　　金：国家自然科学基金(批准号:12175126,11775134)资助的课题.

摘　　要：肌酐是肾脏疾病诊断和监测的关键生物标志物,因此,快速、灵敏的肌酐检测非常重要.本文提供了一种通过提高低温下的光子诱导电荷转移效率来促进表面增强拉曼散射光谱(SERS)活性的有效策略.采用种子生长法获得纳米金二十面体(Au_(20)),以此作为SERS活性基底.采用极低温(98 K)SERS检测技术实现对染料分子结晶紫(CV)和生理盐水中的肌酐含量的快速、灵敏检测.实验结果表明:常温296 K下,Au_(20)基底对CV分子的检测限(LOD)低至10^(-12) mol/L,并且信号均匀;低温98 K时,CV分子的LOD可达10^(-14) mol/L,比296 K时降低2个数量级.最后,使用Au20基底对生理盐水中的肌酐进行无标记检测.结果表明,常温296 K时该SERS基底对肌酐的LOD为10^(-6) mol/L,1619 cm^(-1)峰的线性相关系数为0.9839.低温98 K时,肌酐的浓度探测极限低至10^(-8) mol/L,1619 cm^(-1)峰的线性相关系数变为0.9973.可知,低温使肌酐浓度检测限和特征峰线性度都进一步提高.本文的工作为生物医药领域肌酐浓度的精确检测提供了新的思路.Creatinine is a key biomarker for diagnosing and monitoring kidney disease,so rapid and sensitive testing is very important.Raman spectroscopy is particularly suitable for quantitatively detecting the creatinine in the human environment because it is sensitive to subtle changes in the concentration of the analyte.In this work an effective strategy is provided to promote the activity of surface-enhanced Raman scattering spectroscopy by enhancing the photon-induced charge transfer efficiency at low temperature.The nano-gold icosahedron(Au_(20))is obtained by the seed-growing method,which is used as an active substrate for SERS.The ultra-low temperature(98 K)SERS detection technology is used to realize the rapid and sensitive detection of the dye molecule crystal violet(CV)and creatinine in normal saline.The experimental results show that at room temperature of 296 K,the detection limit of Au_(20) substrate for CV molecules is as low as 10^(-12) mol/L,and the signals are uniform;at a low temperature of 98 K,the detection limit of CV molecules can reach 10^(-14) mol/L,which is two orders of magnitude lower than that at 296 K.As a result,the adopted cryogenic temperature can effectively weaken the lattice thermal vibration and reduce the release of phonons,then suppress phonon-assisted non-radiative recombination.So,it will increase the number of photo-induced electrons to participate in the photo-induced charge transfer efficiency.Finally,we perform the label-free detection of creatinine in saline by using an Au_(20) substrate.The results show that the detection limit of the SERS substrate for creatinine is 10^(-6) mol/L at 296 K,and the linear correlation coefficient of the 1619 cm^(-1) peak is 0.9839.At a low temperature of 98 K,the detection limit of creatinine concentration is as low as 10^(-8) mol/L,and the linear correlation coefficient of the 1619 cm^(-1) peak becomes 0.9973.It can be seen that low temperature may further improve the detection limit of creatinine concentration and the linearity of characterist

关 键 词：低温 表面增强拉曼散射 肌酐 超灵敏检测 

分 类 号：R446.1[医药卫生—诊断学] O657.37[医药卫生—临床医学]