急性脑卒中患者肠道菌群研究  

Gut flora in patients with acute stroke

作　　者：徐晓松[1] 蔡祥胜 李瑞莹[1] 官煜彬 陈小明[1] XU Xiao-song;CAI Xiang-sheng;LI Rui-ying;GUAN Yu-bin;CHEN Xiao-ming(Department of Clinical Laboratory,the First Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou,Guangdong 510080,China;不详)

机构地区：[1]广东药科大学附属第一医院检验科,广东广州510080

出　　处：《中国微生态学杂志》2022年第5期562-566,共5页Chinese Journal of Microecology

摘　　要：目的通过粪便标本检测急性脑卒中患者肠道菌群变化情况,探讨急性脑卒中患者肠道菌群结构。方法通过高通量二代测序技术对10例健康者(对照组)及10例急性脑卒中患者(疾病组)粪便样本进行菌群结构测序分析。结果与对照组比较,急性脑卒中患者粪便样本中物种OTU信息量显著增加(P<0.01),菌群多样性指数(Shannon)和物种均一度指数(Evenness)也有所增加但差异无统计学意义(均P>0.05)。门水平上,疾病组患者肠道Bacteroidetes数量较对照组显著增加,Firmicutes数量显著减少(均P<0.05)。属水平上,疾病组患者肠道Bacteroides、Bilophila、Butyricimonas比例较对照组显著升高,而Collinsella、Coprococcus、Clostridium等比例较对照组显著降低(均P<0.05)。结论急性脑卒中患者肠道菌群结构与健康人存在显著差异。Objective To explore the distribution and changes of gut flora in patients with acute stroke through fecal specimen detection,and discuss the composition of gut flora in the patients.Methods Ten healthy individuals(control group)and 10 patients with acute stroke(disease group)were enrolled.The intestinal flora were analyzed by using high-throughput second-generation sequencing.Results Compared with the control group,the number of OTU information in the gut flora of acute stroke patients increased significantly(P<0.01);the diversity index(Shannon)and species homogeneity index(Evenness)also increased,but with no statistical differences(all P>0.05).Compared with the control group,the abundance of Bacteroidetes increased significantly and that of Firmicutes decreased significantly(all P<0.05).Compared with the normal control group,the abundances of Bacteroides,Bilophila and Butyricimonas increased significantly,however,those of Collinsella,Coprococcus and Clostridium decreased significantly(all P<0.05).Conclusion The composition of gut flora in acute stroke patients was significantly different from that in healthy people.

关 键 词：急性脑卒中 肠道菌群 二代测序 

分 类 号：R446[医药卫生—诊断学]