基于胶体金标记米曲霉素的免疫层析试纸法快速筛查SLE  

Colloidal gold labeled AOL immunochromatographic test for rapid detection of SLE

作　　者：赵丹青 李志 李明[1] 王亚东[2] 张晓辉 王若雨[2] 李文哲[1] ZHAO Dan-qing;LI Zhi;LI Ming;WANG Ya-dong;ZHANG Xiao-hui;WANG Ruo-yu;LI Wen-zhe(College of Basic Medical Sciences,Dalian Medical University,Dalian,Liaoning 116044,China;不详)

机构地区：[1]大连医科大学基础医学院,辽宁大连116044 [2]大连大学附属中山医院,辽宁大连116622

出　　处：《中国微生态学杂志》2022年第5期595-598,共4页Chinese Journal of Microecology

基　　金：国家自然科学基金(31870797)。

摘　　要：目的建立基于胶体金标记米曲霉素(AOL)的快速筛查系统性红斑狼疮(SLE)的免疫层析试纸法。方法利用大肠杆菌BL21原核表达特异性识别核心岩藻糖基的AOL基因(FleA),并进行纯化及胶体金标记。利用特异性识别IgG的Protein G以及胶体金标记的AOL共同建立快速筛查SLE的免疫层析试纸法(ICS)。结果成功表达并纯化AOL重组蛋白,并进行胶体金标记;应用胶体金标记AOL的ICS检测SLE患者血清呈阳性反应,而健康对照及其他自身免疫性疾病(类风湿关节炎、IgA肾病和结缔组织病等)呈阴性反应。结论成功建立基于胶体金标记AOL的快速筛查SLE的ICS,为SLE早期筛查提供了可靠依据。Objective To establish a new rapid method for the diagnosis of systemic lupus erythematosus(SLE)based on AOL-conjugated colloid gold immunochromatographic strips(ICS).Methods AOL gene(FleA)was expressed by using E.coli prokaryote BL21.AOL was purified and connected with colloidal gold.The ICS for rapid detection of SLE based on Protein G that specifically recognizes IgG and AOL-conjugated colloid gold was established.Results AOL was successfully expressed and purified,and then labeled with colloidal gold.The ICS test using colloidal gold connected to AOL showed a positive reaction in the serum of SLE patients.Healthy controls and other autoimmune diseases(rheumatiod arthritis,IgA nephropathy and connective tissue disease)showed negative results.Conclusion The AOL-conjugated colloid gold ICS for rapid detection of SLE was successfully established,which provides a reliable basis for early detection of SLE.

关 键 词：核心岩藻糖基化 免疫层析试纸法 系统性红斑狼疮 

分 类 号：R331[医药卫生—人体生理学]