重组人角质细胞生长因子2对辽宁绒山羊血清KGF-2浓度的影响  

Effect of rhKGF-2 on level of serum KGF-2 in Liaoning Cashmere goat

作　　者：孙亚波 朱延旭 冀红芹 贾富勃[1] 王春艳 吉尚雷[1] 全治国[3] 刘媛缘 Sun Yabo;Zhu Yanxu;Ji Hongqin;Jia Fubo*;Wang Chunyan;Ji Shanglei;Quan Zhiguo;Liu Yuanyuan(Department of Animal Science and Technology,Liaoning Agricultural College,Liaoning Yingkou 115009;Liaoning Province Modern Agricultural Production Base and Construction Engineering Center,Liaoning Shenyang 110032;Corporation of Liaoning Cashmere Goat Original Farm of Liaoning Province,Liaoning Yingkou 115200)

机构地区：[1]辽宁农业职业技术学院,辽宁营口115009 [2]辽宁省现代农业生产基地建设工程中心,辽宁沈阳110032 [3]辽宁省辽宁绒山羊原种场有限公司,辽宁营口115200

出　　处：《现代畜牧兽医》2022年第6期11-17,共7页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：辽宁省自然基金项目“角质细胞生长因子对辽宁绒山羊绒毛生长调控的研究(2020-MS-347)”;国家绒毛用羊产业技术体系饲料资源开发岗位科学家项目(CARS-39-10)。

摘　　要：研究旨在探讨不同处理方式给药后羊血清角质细胞生长因子2 (KGF-2)的浓度变化趋势,为重组人角质细胞生长因子2 (rhKGF-2)调控羊绒生长筛选合适的处理方法和剂量提供参考。试验选择24只5~7月龄、体重为(25.76±1.94) kg的辽宁绒山羊,公、母各半,随机分为4组,每组6个重复,每个重复1只羊。以皮下注射方式,试验1组注射rhKGF-2胶原蛋白溶液,试验2组注射rhKGF-2温敏感凝胶溶液,试验3组注射rhKGF-2生理盐水溶液,试验4组为对照组,不做rhKGF-2处理。共设3个试验周期,每个周期18 d;依次设置低、中、高(10、20、30μg/kg)等3个剂量,每个周期开始时一次性注射18 d用量;研究不同给药方式和剂量对注射后血清KGF-2浓度变化的影响,探讨适宜的给药方式和剂量。结果显示:以胶原蛋白溶液和温敏感凝胶溶液形式皮下注射rhKGF-2后,血清KGF-2浓度先增加后降低,呈近似抛物线形式变化;以生理盐水溶液形式给药后,血清KGF-2浓度迅速升高达到峰值后断崖式直线下降。随着处理剂量增加,3个试验组血清KGF-2浓度峰值出现的时间均向后延迟,KGF-2高浓度维持时间延长;在30μg/kg的剂量时,试验1组的上述两个时间参数为90和426 h,试验2组为138和426 h,试验3组为42和138 h。对于同一种处理方式,剂量增加可使血清KGF-2浓度绝对值增加。研究表明,通过外源供给方式给绒山羊补充KGF-2,增加供给剂量能够提高血清KGF-2绝对浓度、延迟峰值出现时间、延长高浓度维持时间;以胶原蛋白溶液和温敏感凝胶的方式补充KGF-2效果相近,但考虑到可操作性,以前者的效果更好。The purpose of the study was to investigate the changing trend of the concentration of KGF-2 in sheep serum after different treatments and to provide reference for screening of appropriate treatment methods and doses for rhKGF-2 to regulate cashmere growth. Twenty-four Liaoning Cashmere goats aged 5~7 months and weighing(25.76±1.94) kg, half in male and half female, were randomly divided into four groups with six repelicates in each group,and one goat in each repelicate. By subcutaneous injection, group 1 was injected with rh KGF-2 collagen solution, group 2 was injected with rh KGF-2 temperature sensitive gel solution, group 3 was injected with rh KGF-2 physiological saline solution, and group 4 was the control group without rh KGF-2 treatment. There were three periods lasted 18 d in the research which was given 10, 20, 30 μg/kg. The object of the research was finding the effects of different method and quantity of rh KGF-2 on KGF-2 serum level in Liaoning Cashmere goats, getting better method and quantity. The results showed that after subcutaneous injection of rh KGF-2 in the form of collagen solution and temperature-sensitive gel solution, the serum KGF-2 concentration first increased and then decreased, changing in an approximate parabolic form. After administration in the form of normal saline solution, the serum KGF-2 concentration increased rapidly, reached a peak, and then fell off a cliff. With the increase of the treatment dose, the peak time of serum KGF-2 concentration in the three experimental groups was delayed, and the maintenance time of high rh KGF-2 concentration was prolonged. At the dose of 30 μg/kg, the above two time parameters were 90 and 426 h in group 1,138 and 426 h in group 2, 42 and 138 h in group 3. For the same treatment, increasing the dose can increase the absolute value of serum KGF-2 concentration. The study indicates that supplementing KGF-2 to cashmere goats by exogenous supply, increasing the supply dose can increase the absolute concentration of serum KGF-2, delay the tim

关 键 词：角质细胞生长因子2 辽宁绒山羊 重组人角质细胞生长因子2 

分 类 号：S859[农业科学—临床兽医学]