山东新分布药用植物碎米桠的DNA条形码分子鉴定  

Molecular identification of a newly discovered medicinal plant,Isodon rubescens,in Shandong using DNA barcoding

作　　者：葛俊伟 李平[2] 张童童 高飞 王文越 王钧楠 孙稚颖[1] GE Jun-wei;LI Ping;ZHANG Tong-tong;GAO Fei;WANG Wen-yue;WANG Jun-nan;SUN Zhi-ying(Shandong University of Traditional Chinese Medicine, Jinan 250355, China;Beijing University of Traditional Chinese Medicine, Beijing 102488,China)

机构地区：[1]山东中医药大学,山东济南250355 [2]北京中医药大学,北京102488

出　　处：《山东科学》2022年第4期15-20,共6页Shandong Science

基　　金：山东省中医药科技发展计划(2017-516,2019-0973);2017年中医药公共卫生服务补助专项(财社(2017)66号);2021年山东省林业改革发展资金专项(鲁财预指(2021)1号)。

摘　　要：利用DNA条形码技术,对一种在山东省中药资源普查中发现的形态鉴定较困难的植物进行了分子鉴定。首先对样本进行总DNA提取,核ITS2序列、叶绿体psbA-trnH序列的PCR扩增和测序,然后将所得序列用Chromasv校对拼接,利用软件MEGA6.0分析数据。结果表明,该物种为山东新分布唇形科香茶菜属植物碎米桠。该研究为山东省新分布种碎米桠的确定及其资源开发提供了重要证据。DNA barcoding method was carried out to identify a species,which was discovered during field medicinal resource investigation in Shandong and difficult to identify using morphological characters.First,the total DNA was extracted from the samples.Then,the sequences of internal transcribed spacer region 2 and psbA-trnH were amplified and sequenced using polymerase chain reaction.Next,the obtained sequences were proofread and spliced using Chromasv,and the software MEGA 6.0 was used to analyze the data.Consequently,the studied species was identified as Isodon rubescen(Hemsl.)Hara using the DNA barcoding method.This study provides important evidence regarding the identification of a newly discovered species,Isodon rubescens,in Shandong and its medicinal resource development.

关 键 词：山东新分布 碎米桠 DNA条形码 分子鉴定 

分 类 号：R282.5[医药卫生—中药学]