唾液细胞外囊泡miRNA-143/145与雌激素缺乏性骨质疏松相关性的临床研究  

Clinical study on correlation between miRNA-143/145 in saliva and estrogen-deficient osteoporosis

作　　者：沙莎 陆玲波 郭培培 陈姝 靳牧涵 徐荣耀 SHA Sha;LU Lingbo;GUO Peipei;CHEN Shu;JIN Muhan;XU Rongyao(Jiangsu Prouince Key Laboratory of Oral Diseases,Nanjing Medical Unitersily,Nanjing 210029,China)

机构地区：[1]江苏省口腔疾病研究重点实验室,江苏南京210029 [2]江苏省口腔转化医学工程研究中心,江苏南京210029 [3]南京医科大学附属口腔医院口腔颌面外科,江苏南京210029

出　　处：《口腔医学》2022年第6期515-520,共6页Stomatology

基　　金：国家自然科学基金(82001012);中国博士后科学基金批面上资助(2021M691640);江苏省高校自然科学研究面上项目(20KJB320019);江苏省高等学校大学生创新创业训练计划(202110312049Y)。

摘　　要：目的研究唾液细胞外囊泡miRNA-143/145表达水平与女性绝经后骨质疏松的相关性,以探讨其对雌激素缺乏性骨质疏松的诊断价值。方法选取在我院进行种植牙手术的绝经前后女性患者,采用CBCT对松质骨进行骨参数分析,测量患者骨密度差异;选择在我院进行住院手术的绝经期前后女性,分别收集血清、唾液样本,提取细胞外囊泡;透射电镜观察血清和唾液中细胞外囊泡形态;ELISA检测血清中雌激素表达水平;qRT-PCR检测血清和唾液细胞外囊泡中miRNA-143/145水平。结果经分析发现,绝经后女性雌激素水平明显下降,并伴有骨密度的显著降低;经透射电镜观察,血清和唾液中细胞外囊泡为直径约100 nm的杯状双层膜样结构;和绝经前相比,绝经后女性血循环中miRNA-143/145的表达显著升高;进一步检测唾液中miRNA-143/145表达,呈现相似的趋势。结论基于唾液细胞外囊泡中miRNA-143/145检测结果与血清检测相似,提示唾液检测作为一种无创性操作方式,更便于临床医生为女性绝经后骨质疏松状态做出诊断。Objective To investigate the correlation between postmenopausal osteoporosis and the level of miRNA-143/145 derived from extracellular vesicles in saliva,and to explore the diagnostic value of miRNA-143/145 for estrogen deficient osteoporosis.Methods Bone parameters of cancellous bone in premenopausal and postmenopausal female patients undergoing dental implant surgery in our hospital were analyzed by CBCT,and BMD differences were measured.CBCT,serum and saliva samples were collected from premenopausal and postmenopausal women undergoing inpatient surgery in our hospital,and extracellular vesicles were extracted.The morphology of extracellular vesicles in serum and saliva were observed by transmission electron microscopy.The expression level of estrogen in serum was detected by ELISA.qRT-PCR was used to detect miRNA-143/145 levels in extracellular vesicles of serum and saliva.Results After analysis,it was found that the level of estrogen in postmenopausal women decreased significantly,accompanied by a significant decrease in bone mineral density.The extracellular vesicles in serum and saliva observed by transmission electron microscopy(TEM)were cup-shaped bilayer membranous structure about 100 nm in diameter.Compared with premenopausal women,the expression of miRNA-143/145 in blood circulation of postmenopausal women was significantly increased.Further detection of miRNA-143/145 expression in saliva showed a similar trend.Conclusion Results of miRNA-143/145 in extracellular vesicles in saliva are in good agreement with results in serum,which suggests that saliva test,as a noninvasive method,is more convenient for clinicians to diagnose postmenopausal osteoporosis.

关 键 词：雌激素缺乏性骨质疏松 血清 唾液 细胞外囊泡 miRNA-143/145 

分 类 号：R681.4[医药卫生—骨科学]