宿半夏悬浮细胞培养及体细胞胚发生的研究  

Research on Suspension Cell Culture and Somatic Embryogenesis of Pinellia Ternate

作　　者：陶兴魁 张兴桃 冯凡 王海潮 TAO Xingkui;ZHANG Xingtao;FENG Fan;WANG Haichao(School of Biological and Food Engineering,Suzhou University,Suzhou 234000,China)

机构地区：[1]宿州学院生物与食品工程学院,安徽宿州234000

出　　处：《宿州学院学报》2022年第6期31-35,共5页Journal of Suzhou University

基　　金：安徽省高校自然科学研究项目(KJ2019A0665,KJ2017ZD36);宿州学院博士科研启动基金(2019jb22);宿州学院科研平台开放项目(2020ykf20,2021XJPT34)。

摘　　要：为建立宿半夏悬浮细胞培养体系,研究宿半夏悬浮细胞体细胞胚的发生,将半夏愈伤组织接种在液体培养基MS+1.5 mg/L 2,4-D+0.5 mg/L 6-BA+300 mg/L CH中振荡培养,诱导体细胞胚胎的发生。经3~4次继代培养后,悬浮细胞启动分裂的时间逐渐缩短,分裂增生迅速,生长量逐渐增大,过滤可得到单细胞的悬浮系。降低或停止振荡后,产生大量的球形胚,可进一步发育形成成熟的体细胞胚。A suspension cell culture system of Pinellia ternate is established to study the somatic embryogenesis in suspension cells.The calli of Pinellia ternate were inoculated in liquid medium MS+1.5mg/L 2,4-D+0.5mg/L 6-BA+300mg/L CH for oscillating culture to induce somatic embryogenesis.After 3~4 times of subculture,the time for starting the division of the suspension cells is gradually shortened,and the division and proliferation are rapid.The growth volume is gradually increased,and the single-cell suspension line can be obtained by filtering.After the oscillation is reduced or stopped,a large number of spherical embryos are produced,which can further develop into mature embryos.

关 键 词：宿半夏 悬浮培养 单细胞 体细胞胚 

分 类 号：S663.9[农业科学—果树学]