三种近交系小鼠自发行为图谱的构建与比较  

Construction and Comparison of the Spontaneous Behavior Atlas in Three Inbred Mice

作　　者：李笑溪 韩亚宁 黄康[1,2] 任真 王立平 朱英杰[1] LI Xiaoxi;HAN Yaning;HUANG Kang;REN Zhen;WANG Liping;ZHU Yingjie(Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院深圳先进技术研究院,深圳518055 [2]中国科学院大学,北京100049

出　　处：《集成技术》2022年第4期70-79,共10页Journal of Integration Technology

基　　金：国家自然科学基金青年基金项目(31800886);国家自然科学基金重点项目(31930047);广东省重点领域研发计划项目(2018B030331001)。

摘　　要：小鼠被广泛应用于各类生理、病理和行为学实验。不同基因型小鼠的自发行为差异对实验设计和结果的影响尚未明确。该文选择目前常用的3种近交品系小鼠C57BL/6J、C57BL/6N、BALB/c(简称6J、6N、BC),利用基于层次化三维动物行为特征的无监督学习框架,对其自发行为进行了客观评估。实验结果表明,基因型显著影响小鼠的自发行为,影响因素造成的差异强度为:远基因型>近基因型>雌雄对照。具体表现为,BC与6J和6N的体态明显不同,且在“抬头”“梳理”“跑”3种运动模式上,BC行为明显异于6J和6N。6J和6N的行为谱虽较为类似,但6N的嗅探行为明显多于6J。该研究结果可作为小鼠模型实验设计、品系选择和样本量确定的参考。Mice are widely used in various physiological,pathological and behavioral experiments.However,it is yet unclear whether or how much spontaneous behavior varies between mice with different genotypes,which may affect experiment design and outcome.In this study,three inbred mice C57BL/6J,C57BL/6N,BALB/c(6J,6N,BC)were selected,and objectively evaluated for their spontaneous behavior with a hierarchical unsupervised learning framework for 3D animal behavioral characteristics.The results showed that genotype is the most significant factors influencing mice,with the whole list presented as distant genotype>near genotype>gender difference.Behavior atlas varies greatly between BC and 6J/6N groups due to obvious differences in body posture,and BC have significantly lower movement speed than 6J and 6N.Although 6J and 6N behavior atlas were relatively similar,6N sniffed significantly than 6J,and the anxiety levels at 6N were relatively high.The results of this study can be used as a reference for experiment design,strain selection,and determination of N value in future research using mouse model.

关 键 词：小鼠 自发行为 3D运动捕获 行为表型 

分 类 号：Q189[生物学—神经生物学]