Molecular phylogeographic analyses and species delimitations reveal that Leopoldamys edwardsi(Rodentia:Muridae)is a species complex  

作　　者：Haotian LI Lingming KONG Kaiyun WANG Shuping ZHANG Masaharu MOTOKAWA Yi WU Wenquan WANG Yuchun LI 

机构地区：[1]Marine College,Shandong University,Weihai,China [2]Institute of Ecology and Biodiversity,Shandong University,Jinan,China [3]The Kyoto University Museum,Kyoto University,Kyoto,Japan [4]College of Life Science,Guangzhou University,Guangzhou,China [5]Institute of Tropical Biosciences and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,China

出　　处：《Integrative Zoology》2019年第5期494-505,共12页整合动物学（英文版）

基　　金：the Major International(Regional)Joint Research Project of NSFC(Grant No.31110103910);the Japan Society for the Promotion of Science(JSPS)Core-to-Core Program B Asia-Africa Scientific Platforms,and the JSPS Grants-in-Aid for Scientific Research(26304009).

摘　　要：Leopoldamys edwardsi is a species with wide distribution ranges in southern China but is not discussed in studies on geographic variation and species differentiation.We used 2 mitochondrial(Cytb,CO1)and 3 nuclear(GHR,IRBP and RAG1)genes to clarify species phylogeography and geographical differentiation.Maximum likelihood(ML)and Bayesian phylogenetic inference(BI)trees consistently indicated that L.edwardsi is a species complex containing 3 main lineages with high Kimura-2-parameter(K2P)divergences(i.e.lineages LN,LS and LHN)found in the northern and southern China and Hainan Island,respectively.The 3 species delimitation methods,automated barcoding gap discovery,Bayesian poisson tree process analysis and Bayesian phylogenetics and phylogeography,consistently supported the existence of cryptic species.Divergence times among the main lineages were inferred to be during the Pleistocene,with LHN/LS split at 1.33 Ma and LN/(LHN+LS)at 2.61 Ma;the diversifications of L.edwardsi complex might be caused by the rapid uplifts of Tibetan Plateau,paleoclimate change and complex topography.The divergence between LHN and LS was probably related to the separation of Hainan Island from the mainland via the formation of the Qiongzhou Strait.Lineages LN and(LS+LHN)likely diverged due to the Wuyi-Nanling mountain range forming a dispersal barrier.Our results suggested that L.edwardsi complex contains at least 3 distinct species:LHN represents L.hainanensis,endemic to Hainan Island and previously considered as a subspecies L.e.hainanensis;LS represents a cryptic species distributed throughout the southern Chinese continent;and LN represents the nominotypical species L.edwardsi.

关 键 词：ancient hybridization cryptic species Leopoldamys edwardsi complex Leopoldamys hainanensis species delimitation 

分 类 号：Q95[生物学—动物学]