Intervention effect and mechanism of Jisheng Shenqi Decoction plus Panax notoginseng and Bionjia on CCl4-induced hepatic fibrosis rat model  

作　　者：Zhen-Kang Zhong Xiao-Ling Zhou Yue-Ming Wang Teng Wu Shi-Yin Lu Xin-Hui Shen 

机构地区：[1]Graduate School of Guangxi University of Traditional Chinese Medicine,Nanning 530001,China [2]Liuzhou Hospital of Traditional Chinese Medicine,Liuzhou 545001,China

出　　处：《Journal of Hainan Medical University》2022年第10期20-26,共7页海南医学院学报（英文版）

基　　金：National Natural Science Foundation of China(No.81760855);Liuzhou City Liver Disease Clinical Research Center Project(No.2018AF10503);Guangxi Postgraduate Education Innovation Program(No.YCSY2020106)。

摘　　要：Objective:To investigate the intervention effect and specific mechanism of Jisheng Shenqi Decoction plus Panax notoginseng and Turmeric on carbon tetrachloride(CCl4)-induced liver fibrosis in rats.Methods:SPF SD rats were randomly divided into control group,model group,and Chinese medicine treatment(low,medium and high dose)groups.The model group and Chinese medicine treatment group were given intraperitoneal injection of 40%CCl4 olive oil solution twice a week.A rat model of liver fibrosis was replicated by the method for 8 weeks.After successful modeling,each drug-administered group was gavaged with corresponding drugs,and the control group and model group were gavaged with equal volume of distilled water,once a day,for 4 weeks.After the last intragastric administration,HE staining and Masson staining were used to observe the pathological morphology of liver tissue,and liver function(ALT,AST)was detected;ELISA method was used to determine transforming growth factorβ(TGF-β),angiotensin II(Ang-Ⅱ),chitinase 3-like protein 1(CHI3L1),interleukin-1(IL-1),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)content;The expression levels of type I,type III collagen(Col-Ⅰ,Col-Ⅲ)and TGF-βmRNA were determined by RT-PCR.Results:①HE and Masson staining showed that a large number of liver cells in the model group were inflamed and necrotic,the collagen fibers in the liver tissue were extensively proliferated,the fibrous septa were interconnected,and the pseudolobules were formed.Compared with the model group,the levels of ALT,AST,TGF-β,Ang-Ⅱ,CHI3L1,IL-1,IL-6 and TNF-αin the traditional Chinese medicine treatment group were decreased,while the levels of Col-Ⅰ,Col-ⅢThe expressions ofⅢand TGF-βmRNA were decreased,and the effect was most obvious in the middle and high dose groups of traditional Chinese medicine(P<0.01).Conclusion:Jisheng Shenqi Decoction combined with Panax notoginseng and Biejia can significantly improve liver fibrosis induced by CCl4 in rats,and its mechanism may be related to the down-reg

关 键 词：Jisheng Shenqi decoction with Panax notoginseng and Turtle shell Liver fibrosis Rats ACE-AngⅡ-AT1R signaling pathway 

分 类 号：R28[医药卫生—中药学]