基于Parkin信号通路研究加味丹参饮对缺氧复氧心肌细胞线粒体自噬的影响  

Effect of Jiawei Danshen decoction on mitophagy in cardiomyocytes with hypoxia/reoxygenation injury: A study based on the Parkin signaling pathway

作　　者：杨艳红 李立 吴若霞 胡方林 YANG Yanhong;LI Li;WU Ruoxia;HU Fanglin(College of Traditional Chinese Medicine,Hunan University of Chinese Medicine,Changsha 410208,Hunan,China)

机构地区：[1]湖南中医药大学中医学院,湖南长沙410208

出　　处：《湖南中医杂志》2022年第6期174-178,共5页Hunan Journal of Traditional Chinese Medicine

摘　　要：目的:通过体外建立大鼠心肌细胞缺氧复氧(HR)损伤模型,观察加味丹参饮(JWDS)能否通过激活Parkin蛋白介导的线粒体自噬而发挥对缺血再灌注心肌的保护作用。方法:将H9C2心肌细胞传代培养后,随机分为5组,分别为正常对照组(NG)、模型组(HRG)、模型组+空白血清处理组(BSG)、模型组+加味丹参饮含药血清组(JWDSG)、模型组+加味丹参饮含药血清+Mdivi-1组(JDMG),应用免疫印迹法比较各组心肌细胞内Parkin蛋白、Beclin-1蛋白的表达情况。结果:与NG组比较,HRG、BSG、JDMG、JWDSG的Parkin、Beclin-1蛋白表达均有所升高,差异均有统计学意义(P<0.05);与BSG、JDMG组比较,JWDSG的Parkin、Beclin-1蛋白表达升高,差异均有统计学意义(P<0.05);HRG、BSG、JDMG 3组之间,以上2项指标比较,差异均无统计学意义(P>0.05)。结论:加味丹参饮可能通过调控Parkin蛋白通路,激活线粒体自噬相关蛋白,发挥减轻心肌缺血再灌注损伤的作用。Objective: To establish a rat cardiomyocyte model of hypoxia/reoxygenation injury in vitro, and to investigate whether Jiawei Danshen decoction(JWDS) can exert a protective effect against hypoxia/reoxygenation injury in myocardium by activating mitophagy mediated by Parkin protein.Methods: H9 C2 cardiomyocytes after subculture were randomly divided into normal control group(NG group),model group(HRG) group, model+blank serum treatment group(BSG group),model+serum containing JWDS group(JWDSG group),and model+serum containing JWDS+Mdivi-1 group(JDMG group),and Western blotting was used to compare the protein expression of Parkin and Beclin-1 in cardiomyocytes between groups.Results: Compared with the NG group, the HRG,BSG,JDMG,and JWDSG groups had significant increases in the protein expression of Parkin and Beclin-1(P<0.05),and compared with the BSG and JDMG groups, the JWDSG group had significant increases in the protein expression of Parkin and Beclin-1(P<0.05),while there were no significant differences in these indices between the HRG,BSG,and JDMG groups(P>0.05).Conclusion: JWDS can alleviate ischemia/reoxygenation injury in myocardium by regulating the Parkin pathway and activating mitophagy-related proteins.

关 键 词：缺氧复氧损伤 加味丹参饮 线粒体自噬 Parkin通路 实验研究 

分 类 号：R285.5[医药卫生—中药学]