三种微藻的生物量与其细胞氮磷碳的相互关系  

Relationship between cell quantity of three microalgae with their carbon,nitrogen and phosphorus

作　　者：曹煜成[1,2,3] 孙志伟 徐煜[1,2,3] 胡晓娟[1,2] 苏浩昌 徐武杰[1,2] 文国樑 鲁敏[1] 余招龙 CAO Yucheng;SUN Zhiwei;XU Yu;HU Xiaojuan;SU Haochang;XU Wujie;WEN Guoliang;LU Min;YU Zhaolong(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment,Guangzhou 510300,China;Shenzhen Base South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shenzhen 518121,China;Maoming Branch of Guandong Laboratory for Lingnan Modern Agriculture,Maoming 525400,China;Guangdong Guanlida Marine Biology Co.,Ltd.,Maoming 525400,China)

机构地区：[1]中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室/广东省渔业生态环境重点实验室,广州510300 [2]中国水产科学研究院南海水产研究所深圳试验基地,广东深圳518121 [3]岭南现代农业科学与技术广东省实验室茂名分中心,广东茂名525400 [4]广东冠利达海洋生物有限责任公司,广东茂名525400

出　　处：《生态科学》2022年第4期204-211,共8页Ecological Science

基　　金：国家重点研发计划(2020YFD0900401);中国水产科学研究院基本科研业务费专项资金(2020TD54);财政部和农业农村部:国家现代农业产业技术体系(CARS-48);广东省现代农业产业技术体系创新团队建设专项资金(2019KJ149);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金项目(2021SD08)。

摘　　要：为明确钝顶螺旋藻(Spirulina platensis)、绿色颤藻(Oscillatoria chlorina)、牟氏角毛藻(Chaetoceros muelleri)等三种池塘水体环境中常见优势微藻的细胞数量与其所含总氮(total nitrogen,TN)、总磷(total phosphorus,TP)和总碳(total carbon,TC)质量之间的数量关系,对以上微藻分别进行纯培养,在明确微藻细胞数量的条件下,将藻细胞冷冻干燥制备为冻干粉,准确测定其干重(cell dry weight,CDW),以及所含TN、TP和TC的质量,并就CDW与其所含TN、TP、TC质量间的数量关系,以及细胞数量与CDW的相互关系等建立数学方程。以上三种微藻的CDW与其所含TN、TP、TC的质量间均存在显著的线性正相关关系(P<0.05),其中在与TN、TP关系的数学回归方程中线性拟合程度优于TC;同时,三种微藻的细胞数量与CDW之间同样呈显著的线性正相关关系(P<0.05)。其中钝顶螺旋藻细胞中的TN、TC、TP含量占其CDW的比值分别为29.3%、13.6%和4.4%,均远高于绿色颤藻和牟氏角毛藻。In order to evaluate the relationship between the cell quantity and the contents of total nitrogen(TN),total phosphorus(TP)and total carbon(TC)in Spirulina platensis,Oscillatoria chlorine and Chaetoceros muelleri,which were dominant microalgal species in aquaculture pond,the microalgae were pure cultured in biological incubator with constant temperature and illumination respectively,and cell quantity were determined continuously.Microalgal cells were collected by centrifugation and freeze-dried to prepare dry powder,the cells dry weight(CDW)and the contents of TN,TP and TC also were accurately measured.Based on the measured data,the mathematical equations were established on quantitative relationship between CDW and the contents of TN,TP and TC,as well as the relationship between microalgal cell quantity and CDW.There was significant linear positive correlation between CDW of the three microalgae with their contents of TN,TP and TC(P<0.05);the linear fit degree in the mathematical regression equation of TN and TP was better than that of TC;meanwhile,the cell quantity of three microalgae also was linear positive correlation with CDW significantly(P<0.05).The ratio of TN,TC and TP to CDW in S.platensis cells was 29.3%,13.6%and 4.4%respectively,which were much higher than those of O.chlorine and C.muelleri.

关 键 词：钝顶螺旋藻 牟氏角毛藻 绿色颤藻 总氮 总磷 总碳 

分 类 号：X703.1[环境科学与工程—环境工程]