短花针茅StbCO和StbFT基因全长的克隆与表达差异分析  

Cloning and Differential Expression Analysis of Full-lengthStbCO and StbFT Genes in Stipa breviflora

作　　者：赵鸿彬[1,2,3,4] 范宇凤 张晓馥 范月月 张涛 白雪[1] ZHAO Hong-bin;FAN Yu-feng;ZHANG Xiao-fu;FAN Yue-yue;ZHANG Tao;BAI Xue(College of Life Science,Inner Mongolia Agricultural University,Hohhot 010018,China;Key Laboratory of Biological Manufacturing of Inner Mongolia Autonomous Region,Hohhot 010018,China;Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology,Hohhot 010018,China;Key Lab of Germplasm Innovation and Utilization of Triticeae Crop at Universities of Inner Mongolia Autonomous Region,Hohhot 010018,China;Architectural Engineering Institute,Taishan College of Science and Technology,Taian 271038,China)

机构地区：[1]内蒙古农业大学生命科学学院,内蒙古呼和浩特010018 [2]内蒙古自治区生物制造重点实验室,内蒙古呼和浩特010018 [3]内蒙古自治区植物逆境生理与分子生物学重点实验室,内蒙古呼和浩特010018 [4]内蒙古高校麦类作物种质创新与利用重点实验室,内蒙古呼和浩特010018 [5]泰山科技学院建筑工程学院,山东泰安271038

出　　处：《中国草地学报》2022年第7期1-12,共12页Chinese Journal of Grassland

基　　金：国家自然科学基金项目(31760154);内蒙古自然科学基金项目(2021MS03094)。

摘　　要：光周期调控在植物从营养生长到生殖生长的转变过程中起重要作用,CO和FT基因位于光周期途径的关键位点。参照转录组数据对响应短花针茅开花的主要调控基因StbCO和StbFT基因进行了克隆、亚细胞定位和表达分析,研究短花针茅植物开花与气候变化的响应规律。结果表明,StbCO和StbFT基因全长分别为2065bp和1389bp,分别包含1179bp和534bp的完整编码区。亚细胞定位发现,StbCO蛋白位于细胞核,StbFT蛋白分布于细胞质和细胞核。增温和施氮处理下,StbCO和StbFT基因在短花针茅开花的不同时期表达量有较大差异,为进一步解析其在短花针茅开花过程中的功能提供了有用的信息。Photoperiod regulation plays an important in the transformation of plants from vegetative growth to reproductive growth.CO and FT genes are key regulatory sites in the photoperiod pathway.In this paper,we cloned,subcellular located and expressed StbCO and StbFT genes,the main regulatory genes of Stipa breviflora in response to flowering,with reference to transcriptome data,in order to study the response of Stipa breviflora to flowering and climate change.The results showed that the full length of StbCO and StbFT genes were 2065bp and 1389bp,respectively,including 1179bp and 534bp complete coding regions.Analysis of subcellular localization revealed that StbCO protein was located in the nucleus,while StbFT protein was distributed in the cytoplasm and nucleus.The expression levels of StbCO and StbFT genes were significantly different at different flowering stages of Stipa breviflora under warming and nitrogen application treatments,which provided useful information for further analysis of their functions in the flowering process of Stipa breviflora.

关 键 词：短花针茅 StbCO StbFT 基因克隆 表达分析 

分 类 号：Q943.2[生物学—植物学] S543.9[农业科学—作物学]